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Western blot of ICAM-4/CD242 in human umbillical cord lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with an ICAM-4/CD242 polyclonal antibody (Product# PA5-37869) at a dilution of 1ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-RHGSRVIYSESLER, from the internal region of ICAM4 (aa169-182)|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD242; CD242 antigen; ICAM-4; ICAM4; Intercellular adhesion molecule 4; intercellular adhesion molecule 4 (LW blood group); intercellular adhesion molecule 4 isoform 3 anitgen LWa; Landsteiner-Wiener blood group; Landsteiner-Wiener blood group antigen a; Landsteiner-Wiener blood group glycoprotein; LW; LW blood group protein
CD242; ICAM4; LW