Proteintech

IDE Polyclonal Antibody

View all (30) IDE antibodies

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IDE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens).

IDE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens).

IDE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens).

IDE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens).

IDE Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IDE Antibody in Western Blot (WB) — A2780 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

IDE Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

IDE Antibody in Immunoprecipitation (IP) — IP result of anti-IDE (IP:21728-1-AP, 4ug; Detection:21728-1-AP 1:500) with HepG2 cells lysate 1600ug.

Product Details

21728-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 6 publications 6 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IDE Fusion Protein Ag16392 (676-1019 aa encoded by BC096336) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is specific to IDE.

Immunogen sequence: EQPHQHAMY YLRLLMTEVA WTKDELKEAL DDVTLPRLKA FIPQLLSRLH IEALLHGNIT KQAALGIMQM VEDTLIEHAH TKPLLPSQLV RYREVQLPDR GWFVYQQRNE VHNNCGIEIY YQTDMQSTSE NMFLELFCQI ISEPCFNTLR TKEQLGYIVF SGPRRANGIQ GLRFIIQSEK PPHYLESRVE AFLITMEKSI EDMTEEAFQK HIQALAIRRL DKPKKLSAEC AKYWGEIISQ QYNFDRDNTE VAYLKTLTKE DIIKFYKEML AVDAPRRHKV SVHVLAREMD SCPVVGEFPC QNDINLSQAP ALPQPEVIQN MTEFKRGLPL FPLVKPHINF MAAKL (676-1019 aa encoded by BC096336)

Target Information

This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing β-amyloid clearance in APP/PS1 mice.

Biochimica et biophysica acta. Molecular basis of disease

Yuan C,Guo X,Zhou Q,Du F,Jiang W,Zhou X,Liu P,Chi T,Ji X,Gao J,Chen C,Lang H,Xu J,Liu D,Yang Y,Qiu S,Tang X,Chen G,Zou L

Tue Jan 01 00:00:00 EST 2019

Ginsenoside Compound K Regulates Amyloid β via the Nrf2/Keap1 Signaling Pathway in Mice with Scopolamine Hydrobromide-Induced Memory Impairments.

Journal of molecular neuroscience : MN

Yang Q,Lin J,Zhang H,Liu Y,Kan M,Xiu Z,Chen X,Lan X,Li X,Shi X,Li N,Qu X

Tue Jan 01 00:00:00 EST 2019

A pectin from fruits of Lycium barbarum L. decreases β-amyloid peptide production through modulating APP processing.

Carbohydrate polymers

Zhou L,Liao W,Zeng H,Yao Y,Chen X,Ding K

Sat Dec 01 00:00:00 EST 2018

Novel Serum Biomarkers Detected by Protein Array in Polycystic Ovary Syndrome with Low Progesterone Level.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Zheng Q,Zhou F,Cui X,Liu M,Li Y,Liu S,Tan J,Yan Q

Fri Aug 17 00:00:00 EDT 2018

Effect of naringenin on brain insulin signaling and cognitive functions in ICV-STZ induced dementia model of rats.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology

Yang W,Ma J,Liu Z,Lu Y,Hu B,Yu H

Thu May 01 00:00:00 EDT 2014

Cell surface expression of the major amyloid-β peptide (Aβ)-degrading enzyme, neprilysin, depends on phosphorylation by mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) and dephosphorylation by protein phosphatase 1a.

The Journal of biological chemistry

Kakiya N,Saito T,Nilsson P,Matsuba Y,Tsubuki S,Takei N,Nawa H,Saido TC

Fri Aug 24 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Abeta-degrading protease; FLJ35968; Insulin degrading enzyme; Insulin protease; Insulin-degrading enzyme; Insulinase; Insulysin

Gene Aliases: IDE; INSULYSIN

UniProt ID: (Human) P14735

Entrez Gene ID: (Human) 3416

Molecular Function: metalloprotease

proteolysis insulin receptor signaling pathway determination of adult lifespan bradykinin catabolic process ubiquitin homeostasis protein processing positive regulation of protein oligomerization hormone catabolic process negative regulation of proteolysis viral entry into host cell beta-amyloid metabolic process protein homooligomerization protein homotetramerization protein heterooligomerization proteolysis involved in cellular protein catabolic process insulin metabolic process insulin catabolic process

Process(es)

beta-amyloid binding virus receptor activity glycoprotein binding metalloendopeptidase activity receptor binding protein binding ATP binding zinc ion binding ATPase activity beta-endorphin binding peptide binding protein homodimerization activity ubiquitin binding insulin binding