Invitrogen

IDE Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

2 References

View all (30) IDE antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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IDE Antibody in Immunocytochemistry (ICC/IF) — IDE Polyclonal Antibody detects IDE protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IDE stained by IDE Polyclonal Antibody (Product # PA5-29350) diluted at 1:500. Blue: Fluoroshield with DAPI .

IDE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — IDE Polyclonal Antibody detects IDE protein at cytosol on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. IDE Polyclonal Antibody (Product # PA5-29350) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

IDE Antibody in Western Blot (WB) — Western Blot analysis of IDE was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a IDE Polyclonal Antibody (Product # PA5-29350) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

IDE Antibody in ELISA (ELISA) — Published figure using IDE polyclonal antibody (Product # PA5-29350) in ELISA

IDE Antibody — Figure 3. Expression of pathological factors in 3xTg-AD model mice following treatment with Simvastatin. ELISA assays were performed and determine that (A) Simvastatin treatment reduced intracellular levels of Abeta-42 and Abeta-40 peptides in the cerebrospinal fluid. Furthermore, (B) Simvastatin treatment downregulated IL-1beta and MCP-1 in the cerebrospinal fluid and (C) Simvastatin treatment upregulated neprilysin and insulin in the cerebrospinal fluid. Additonally, (D) Simvastatin treatment upregulated IGFBP-3 and VEGF-beta in the cerebrospinal fluid. Data are represented as the mean +- standard error of the mean (n=3 per group). **P<0.01 vs. control. PBS, phosphate-buffered saline; 3xTg-AD, triple-transgenic Alzheimer's disease; Abeta, amyloid beta; IL, interleukin; MCP, monocyte chemoattractant protein; IGFBP, insulin-like growth factor-binding protein; VEGF, vascular endothelial growth factor. {TM}

Product Details

PA5-29350

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:20,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
ELISA (ELISA)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 349 and 635 of Human insulin degrading enzyme View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 10% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2546826

Product Specific Information

Recommended positive controls: A431, H1299, HeLa, HepG2, mouse brain, rat brain.

Predicted reactivity: Mouse (94%), Rat (95%), Zebrafish (90%), Dog (93%), Rhesus Monkey (99%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Abelmoschus esculentus subfractions attenuate Aβ and tau by regulating DPP-4 and insulin resistance signals.

BMC complementary medicine and therapies

Huang CN,Wang CJ,Lin CL,Li HH,Yen AT,Peng CH

PA5-29350 was used in Western Blotting to investigate whether Abelmoschus esculentus could be effective to attenuate beta amyloid generation and metabolism, via regulating DPP4 and insulin resistance cascades.