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Invitrogen
IDE Polyclonal Antibody
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FIGURE: 1 / 5
IDE Antibody (PA5-79435) in IHC (P)
Immunohistochemistry analysis of IDE on paraffin-embedded human mammary cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with IDE polyclonal antibody (Product# PA5-79435) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Dev... View More
Product Details
PA5-79435
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human IDE recombinant protein (Position: F485-K756).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human Caco-2 whole cell, rat brain tissue, mouse brain tissue. IHC: human mammary cancer tissue, rat pancreas tissue. Flow: Hela cell, CACO-2 cell.
Target Information
This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Abeta-degrading protease; FLJ35968; Insulin degrading enzyme; Insulin protease; Insulin-degrading enzyme; Insulinase; Insulysin
Gene Aliases: 1300012G03Rik; 4833415K22Rik; AA675336; AI507533; IDE; INSDEGM; INSULYSIN
UniProt ID: (Human) P14735, (Rat) P35559
Entrez Gene ID: (Human) 3416, (Rat) 25700, (Mouse) 15925
beta-amyloid binding
virus receptor activity
glycoprotein binding
metalloendopeptidase activity
receptor binding
protein binding
ATP binding
zinc ion binding
ATPase activity
beta-endorphin binding
peptide binding
protein homodimerization activity
ubiquitin binding
insulin binding
peptidase activity
peptide hormone binding
nucleotide binding
catalytic activity
metallopeptidase activity
hydrolase activity
metal ion binding
metalloprotease
proteolysis
insulin receptor signaling pathway
determination of adult lifespan
bradykinin catabolic process
ubiquitin homeostasis
protein processing
positive regulation of protein oligomerization
hormone catabolic process
negative regulation of proteolysis
viral entry into host cell
beta-amyloid metabolic process
protein homooligomerization
protein homotetramerization
protein heterooligomerization
proteolysis involved in cellular protein catabolic process
insulin metabolic process
insulin catabolic process
peptide catabolic process
cellular protein catabolic process
metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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