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Invitrogen
IDE Polyclonal Antibody
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FIGURE: 1 / 5
IDE Antibody (PA5-79435) in IHC (P)
Immunohistochemistry analysis of IDE on paraffin-embedded human mammary cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with IDE polyclonal antibody (Product# PA5-79435) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Dev... View More
Product Details
PA5-79435
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human IDE recombinant protein (Position: F485-K756).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human Caco-2 whole cell, rat brain tissue, mouse brain tissue. IHC: human mammary cancer tissue, rat pancreas tissue. Flow: Hela cell, CACO-2 cell.
Target Information
This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Abeta-degrading protease; FLJ35968; Insulin degrading enzyme; Insulin protease; Insulin-degrading enzyme; Insulinase; Insulysin; unnamed protein product
Gene Aliases: 1300012G03Rik; 4833415K22Rik; AA675336; AI507533; IDE; INSDEGM; INSULYSIN
UniProt ID: (Human) P14735, (Rat) P35559
Entrez Gene ID: (Human) 3416, (Rat) 25700, (Mouse) 15925
nucleotide binding
beta-amyloid binding
virus receptor activity
catalytic activity
endopeptidase activity
metalloendopeptidase activity
protein binding
ATP binding
peptidase activity
metallopeptidase activity
zinc ion binding
hydrolase activity
ATPase activity
peptide hormone binding
beta-endorphin binding
peptide binding
identical protein binding
protein homodimerization activity
insulin binding
macromolecular complex binding
metal ion binding
glycoprotein binding
receptor binding
ubiquitin binding
metalloprotease
proteolysis
insulin receptor signaling pathway
bradykinin catabolic process
ubiquitin homeostasis
antigen processing and presentation of endogenous peptide antigen via MHC class I
protein catabolic process
positive regulation of protein binding
hormone catabolic process
peptide catabolic process
positive regulation of protein catabolic process
negative regulation of proteolysis
viral entry into host cell
beta-amyloid metabolic process
proteolysis involved in cellular protein catabolic process
beta-amyloid clearance
amyloid-beta clearance by cellular catabolic process
insulin metabolic process
insulin catabolic process
regulation of aerobic respiration
determination of adult lifespan
positive regulation of protein oligomerization
cellular protein catabolic process
protein homooligomerization
protein homotetramerization
protein heterooligomerization
metabolic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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