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Product Details
PA5-120684
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human IFI16 (NP_0013517961)
Conjugate
Form
Liquid
Concentration
3.21 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914256
Product Specific Information
Positive test controls include: HeLa, MCF7, NIH/3T3, THP-1, Mouse ovary, Mouse spleen, Rat ovary. The target is usually found in the following locations: Cytoplasm, Nucleus.
Immunogen sequence: MGKKYKNIVL LKGLEVINDY HFRMVKSLLS NDLKLNLKMR EEYDKIQIAD LMEEKFRGDA GLGKLIKIFE DIPTLEDLAE TLKKEKLKVK GPALSRKRKK EVDATSPAPS TSSTVKTEGA EATPGAQKRK KSTKEKAGPK GSKVSEEQTQ PPSPAGAGMS TAMGRSPSPK TSLSAPPNSS STENPKTVAK CQVTPRRNVL QKRPVIVKVL STTKPFEYET PEMEKKIMFH ATVATQTQFF HVKVLNTSLK EKFNGKKIII ISDYLEYDSL LEVNEESTVS EAGPNQTFEV PNKIINRAKE
Target Information
IFI16 (gamma-inteferon-inducible protein 16) binds double-stranded DNA. It binds preferentially to supercoiled DNA and cruciform DNA structures. It seems to be involved in transcriptional regulation and as a transcriptional repressor. IFI16 may have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. It controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. IFI16 is also involved in innate immune response by recognizing viral dsDNA in the cytosol and nucleus. After binding to viral DNA in the cytoplasm, IFI16 recruits TMEM173/STING and mediates the induction of IFN-beta. It is necessary to activate the IFR3 signaling cascade suring human herpes simplex virus 1 infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibiton of viral immediate-early gene expression and replication. IFI16 is also involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Gamma-interferon-inducible protein 16; IFI 16C; Ifi-16; interferon, gamma-inducible protein 16; interferon-gamma induced protein IFI 16; Interferon-inducible myeloid differentiation transcriptional activator
Gene Aliases: IFI16; IFNGIP1; PYHIN2
UniProt ID: (Human) Q16666
Entrez Gene ID: (Human) 3428
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
double-stranded DNA binding
protein binding
transcription factor binding
poly(A) RNA binding
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
positive regulation of cytokine production
activation of innate immune response
transcription, DNA-templated
autophagy
inflammatory response
cell proliferation
regulation of autophagy
hemopoiesis
myeloid cell differentiation
monocyte differentiation
positive regulation of type I interferon production
positive regulation of interleukin-1 beta production
regulation of gene expression, epigenetic
cellular response to glucose starvation
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
negative regulation of DNA binding
negative regulation of viral genome replication
innate immune response
negative regulation of innate immune response
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
cellular response to ionizing radiation
intrinsic apoptotic signaling pathway by p53 class mediator
activation of cysteine-type endopeptidase activity
negative regulation of cysteine-type endopeptidase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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