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IFIT2 Polyclonal Antibody

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Antibody Testing Data (9)

IFIT2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

IFIT2 Antibody (12604-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 12604-1-AP (IFIT2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Product Image Gallery

IFIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IFIT2 Antibody in Western Blot (WB) — RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12604-1-AP (IFIT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

IFIT2 Antibody in Immunoprecipitation (IP) — IP result of anti-IFIT2 (IP:12604-1-AP, 4ug; Detection:12604-1-AP 1:300) with mouse skin tissue lysate 3200ug.

Product Details

12604-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 35 publications 35 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IFIT2 Fusion Protein Ag3283 (51-399 aa encoded by BC032839)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: ATMCNLLAY LKHLKGQNEA ALECLRKAEE LIQQEHADQA EIRSLVTWGN YAWVYYHMGR LSDVQIYVDK VRHVCEKFSS PYRIESPELD CEEGWTRLKC GGNQNERAKV CFEKALEKKP KNPEFTSGLA IASYRLDNWP PSQNAIDPLR QAIRLNPDNQ YLKVLLALKL HKMREEGEEE GEGEKLVEEA LEKAPGVTDV LRSAAKFYRR KDEPDKAIEL LKKALEYIPN NAYLHCQIGC CYRAKVFQVM NLRENGMYGK RKLLELIGHA VAHLKKADEA NDNLFRVCSI LASLHALADQ YEEAEYYFQK EFSKELTPVA KQLLHLRYGN FQLYQMKCED KAIHHFIEGV (51-399 aa encoded by BC032839)

Target Information

IFIT2 belongs to the IFIT family. It contains 6 TPR repeats. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFITproteins are produced in human body that are supposed to confer immunity against viral infection. These proteins are generally produced during viral infection, Interferon (IFN) treatment, and during pathogen recognition (Pathogen associated molecular pattern recognition) byimmune systemduring infections.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

YTHDF2 Is Downregulated in Response to Host Shutoff Induced by DNA Virus Infection and Regulates Interferon-Stimulated Gene Expression.

Journal of virology

Hesser CR,Walsh D

Thu Mar 30 00:00:00 EDT 2023

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion.

Signal transduction and targeted therapy

Zheng W,Zhou Z,Rui Y,Ye R,Xia F,Guo F,Liu X,Su J,Lou M,Yu XF

Fri Feb 24 00:00:00 EST 2023

MiR-199a-5P promotes osteogenic differentiation of human stem cells from apical papilla via targeting IFIT2 in apical periodontitis.

Frontiers in immunology

Hu J,Huang X,Zheng L,Zhang Y,Zeng H,Nie L,Pang X,Zhang H

Tue Apr 18 00:00:00 EDT 2023

Involvement of CacyBP/SIP in differentiation and the immune response of HaCaT keratinocytes.

Immunobiology

Leśniak W,Bohush A,Maksymowicz M,Piwowarczyk C,Karolak NK,Jurewicz E,Filipek A

Mon May 01 00:00:00 EDT 2023

Rift Valley Fever Virus Nucleoprotein Triggers Autophagy to Dampen Antiviral Innate Immune Responses.

Journal of virology

Zhu X,Guan Z,Fang Y,Zhang Y,Guan Z,Li S,Peng K

Thu Apr 27 00:00:00 EDT 2023

LncRNA RP5-998N21.4 promotes immune defense through upregulation of IFIT2 and IFIT3 in schizophrenia.

Schizophrenia (Heidelberg, Germany)

Guo B,Jiang T,Wu F,Ni H,Ye J,Wu X,Ni C,Jiang M,Ye L,Li Z,Zheng X,Li S,Yang Q,Wang Z,Huang X,Zhao C

Tue Mar 01 00:00:00 EST 2022

Assessing Mitochondrial DNA Release into the Cytosol and Subsequent Activation of Innate Immune-related Pathways in Mammalian Cells.

Current protocols

Bryant JD,Lei Y,VanPortfliet JJ,Winters AD,West AP

Tue Feb 01 00:00:00 EST 2022

METTL3/m⁶A/IFIT2 regulates proliferation, invasion and immunity in esophageal squamous cell carcinoma.

Frontiers in pharmacology

Ge F,Li Z,Hu J,Pu Y,Zhao F,Kong L

Fri Nov 18 00:00:00 EST 2022

METTL3/m⁶A/IFIT2 regulates proliferation, invasion and immunity in esophageal squamous cell carcinoma.

Frontiers in pharmacology

Ge F,Li Z,Hu J,Pu Y,Zhao F,Kong L

Fri Nov 18 00:00:00 EST 2022

METTL3/m⁶A/IFIT2 regulates proliferation, invasion and immunity in esophageal squamous cell carcinoma.

Frontiers in pharmacology

Ge F,Li Z,Hu J,Pu Y,Zhao F,Kong L

Fri Nov 18 00:00:00 EST 2022

METTL3/m⁶A/IFIT2 regulates proliferation, invasion and immunity in esophageal squamous cell carcinoma.

Frontiers in pharmacology

Ge F,Li Z,Hu J,Pu Y,Zhao F,Kong L

Fri Nov 18 00:00:00 EST 2022

Loss of Mitochondrial Protease CLPP Activates Type I IFN Responses through the Mitochondrial DNA-cGAS-STING Signaling Axis.

Journal of immunology (Baltimore, Md. : 1950)

Torres-Odio S,Lei Y,Gispert S,Maletzko A,Key J,Menissy SS,Wittig I,Auburger G,West AP

Thu Apr 15 00:00:00 EDT 2021

The Landscape of the Tumor Microenvironment in Skin Cutaneous Melanoma Reveals a Prognostic and Immunotherapeutically Relevant Gene Signature.

Frontiers in cell and developmental biology

Zhou S,Sun Y,Chen T,Wang J,He J,Lyu J,Shen Y,Chen X,Yang R

Fri Oct 22 00:00:00 EDT 2021

ETV7 limits antiviral gene expression and control of influenza viruses.

Science signaling

Froggatt HM,Harding AT,Chaparian RR,Heaton NS

Tue Jul 13 00:00:00 EDT 2021

Super-enhancer-associated long noncoding RNA RP11-569A11.1 inhibited cell progression and metastasis by regulating IFIT2 in colorectal cancer.

Journal of clinical laboratory analysis

Chen H,Zheng J,Yan L,Zhou X,Jiang P,Yan F

Tue Jun 01 00:00:00 EDT 2021

PML-II regulates ERK and AKT signal activation and IFNα-induced cell death.

Cell communication and signaling : CCS

Meng X,Chen Y,Macip S,Leppard K

Fri Jul 02 00:00:00 EDT 2021

The Small t Antigen of JC Virus Antagonizes RIG-I-Mediated Innate Immunity by Inhibiting TRIM25's RNA Binding Ability.

mBio

Chiang C,Dvorkin S,Chiang JJ,Potter RB,Gack MU

Tue Apr 13 00:00:00 EDT 2021

Human IFIT3 Protein Induces Interferon Signaling and Inhibits Adenovirus Immediate Early Gene Expression.

mBio

Chikhalya A,Dittmann M,Zheng Y,Sohn SY,Rice CM,Hearing P

Tue Dec 21 00:00:00 EST 2021

Murine Ifit3 restricts the replication of Rabies virus both in vitro and in vivo.

The Journal of general virology

Chai B,Tian D,Zhou M,Tian B,Yuan Y,Sui B,Wang K,Pei J,Huang F,Wu Q,Lv L,Yang Y,Wang C,Fu Z,Zhao L

Thu Jul 01 00:00:00 EDT 2021

Murine Ifit3 restricts the replication of Rabies virus both in vitro and in vivo.

The Journal of general virology

Chai B,Tian D,Zhou M,Tian B,Yuan Y,Sui B,Wang K,Pei J,Huang F,Wu Q,Lv L,Yang Y,Wang C,Fu Z,Zhao L

Thu Jul 01 00:00:00 EDT 2021

ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity.

Nature microbiology

Liu G,Lee JH,Parker ZM,Acharya D,Chiang JJ,van Gent M,Riedl W,Davis-Gardner ME,Wies E,Chiang C,Gack MU

Thu Apr 01 00:00:00 EDT 2021

Translation stress and collided ribosomes are co-activators of cGAS.

Molecular cell

Wan L,Juszkiewicz S,Blears D,Bajpe PK,Han Z,Faull P,Mitter R,Stewart A,Snijders AP,Hegde RS,Svejstrup JQ

Thu Jul 01 00:00:00 EDT 2021

Mouse Ifit1b is a cap1-RNA-binding protein that inhibits mouse coronavirus translation and is regulated by complexing with Ifit1c.

The Journal of biological chemistry

Mears HV,Sweeney TR

Fri Dec 18 00:00:00 EST 2020

Cell Cycle Checkpoints Cooperate to Suppress DNA- and RNA-Associated Molecular Pattern Recognition and Anti-Tumor Immune Responses.

Cell reports

Chen J,Harding SM,Natesan R,Tian L,Benci JL,Li W,Minn AJ,Asangani IA,Greenberg RA

Tue Sep 01 00:00:00 EDT 2020

ISG15-dependent Activation of the RNA Sensor MDA5 and its Antagonism by the SARS-CoV-2 papain-like protease.

bioRxiv : the preprint server for biology

Liu G,Lee JH,Parker ZM,Acharya D,Chiang JJ,van Gent M,Riedl W,Davis-Gardner ME,Wies E,Chiang C,Gack MU

Tue Oct 27 00:00:00 EDT 2020

Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs.

Nature microbiology

Tran V,Ledwith MP,Thamamongood T,Higgins CA,Tripathi S,Chang MW,Benner C,García-Sastre A,Schwemmle M,Boon ACM,Diamond MS,Mehle A

Tue Dec 01 00:00:00 EST 2020

Temporal Proteomic Analysis of BK Polyomavirus Infection Reveals Virus-Induced G₂ Arrest and Highly Effective Evasion of Innate Immune Sensing.

Journal of virology

Caller LG,Davies CTR,Antrobus R,Lehner PJ,Weekes MP,Crump CM

Thu Aug 15 00:00:00 EDT 2019

Ifit1 regulates norovirus infection and enhances the interferon response in murine macrophage-like cells.

Wellcome open research

Mears HV,Emmott E,Chaudhry Y,Hosmillo M,Goodfellow IG,Sweeney TR

Sun Jan 10 00:00:00 EST 2021

The histone chaperone HIRA promotes the induction of host innate immune defences in response to HSV-1 infection.

PLoS pathogens

McFarlane S,Orr A,Roberts APE,Conn KL,Iliev V,Loney C,da Silva Filipe A,Smollett K,Gu Q,Robertson N,Adams PD,Rai TS,Boutell C

Fri Mar 01 00:00:00 EST 2019

Ribosome biogenesis restricts innate immune responses to virus infection and DNA.

eLife

Bianco C,Mohr I

Mon Dec 16 00:00:00 EST 2019

Anti-apoptotic effect by the suppression of IRF1 as a downstream of Wnt/β-catenin signaling in colorectal cancer cells.

Oncogene

Ohsugi T,Yamaguchi K,Zhu C,Ikenoue T,Takane K,Shinozaki M,Tsurita G,Yano H,Furukawa Y

Thu Aug 01 00:00:00 EDT 2019

IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA.

Nucleic acids research

Fleith RC,Mears HV,Leong XY,Sanford TJ,Emmott E,Graham SC,Mansur DS,Sweeney TR

Fri Jun 01 00:00:00 EDT 2018

Distinct temporal roles for the promyelocytic leukaemia (PML) protein in the sequential regulation of intracellular host immunity to HSV-1 infection.

PLoS pathogens

Alandijany T,Roberts APE,Conn KL,Loney C,McFarlane S,Orr A,Boutell C

Mon Jan 01 00:00:00 EST 2018

Distinct temporal roles for the promyelocytic leukaemia (PML) protein in the sequential regulation of intracellular host immunity to HSV-1 infection.

PLoS pathogens

Alandijany T,Roberts APE,Conn KL,Loney C,McFarlane S,Orr A,Boutell C

Mon Jan 01 00:00:00 EST 2018

Defining the Role of Stress Granules in Innate Immune Suppression by the Herpes Simplex Virus 1 Endoribonuclease VHS.

Journal of virology

Burgess HM,Mohr I

Wed Aug 01 00:00:00 EDT 2018

Defining the Role of Stress Granules in Innate Immune Suppression by the Herpes Simplex Virus 1 Endoribonuclease VHS.

Journal of virology

Burgess HM,Mohr I

Wed Aug 01 00:00:00 EDT 2018

The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling.

Journal of virology

Chiang C,Pauli EK,Biryukov J,Feister KF,Meng M,White EA,Münger K,Howley PM,Meyers C,Gack MU

Thu Mar 15 00:00:00 EDT 2018

The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling.

Journal of virology

Chiang C,Pauli EK,Biryukov J,Feister KF,Meng M,White EA,Münger K,Howley PM,Meyers C,Gack MU

Thu Mar 15 00:00:00 EDT 2018

PLZF inhibits proliferation and metastasis of gallbladder cancer by regulating IFIT2.

Cell death & disease

Shen H,Zhan M,Zhang Y,Huang S,Xu S,Huang X,He M,Yao Y,Man M,Wang J

Mon Jan 22 00:00:00 EST 2018

PLZF inhibits proliferation and metastasis of gallbladder cancer by regulating IFIT2.

Cell death & disease

Shen H,Zhan M,Zhang Y,Huang S,Xu S,Huang X,He M,Yao Y,Man M,Wang J

Mon Jan 22 00:00:00 EST 2018

Decreased expression of interferon-induced protein 2 (IFIT2) by Wnt/β-catenin signaling confers anti-apoptotic properties to colorectal cancer cells.

Oncotarget

Ohsugi T,Yamaguchi K,Zhu C,Ikenoue T,Furukawa Y

Tue Nov 21 00:00:00 EST 2017

Decreased expression of interferon-induced protein 2 (IFIT2) by Wnt/β-catenin signaling confers anti-apoptotic properties to colorectal cancer cells.

Oncotarget

Ohsugi T,Yamaguchi K,Zhu C,Ikenoue T,Furukawa Y

Tue Nov 21 00:00:00 EST 2017

A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.

Nature immunology

Chan YK,Gack MU

Sun May 01 00:00:00 EDT 2016

A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.

Nature immunology

Chan YK,Gack MU

Sun May 01 00:00:00 EDT 2016

Long non-coding RNA LINC00161 sensitises osteosarcoma cells to cisplatin-induced apoptosis by regulating the miR-645-IFIT2 axis.

Cancer letters

Wang Y,Zhang L,Zheng X,Zhong W,Tian X,Yin B,Tian K,Zhang W

Mon Nov 28 00:00:00 EST 2016

Long non-coding RNA LINC00161 sensitises osteosarcoma cells to cisplatin-induced apoptosis by regulating the miR-645-IFIT2 axis.

Cancer letters

Wang Y,Zhang L,Zheng X,Zhong W,Tian X,Yin B,Tian K,Zhang W

Mon Nov 28 00:00:00 EST 2016

Herpes Simplex Virus 1 Tegument Protein UL41 Counteracts IFIT3 Antiviral Innate Immunity.

Journal of virology

Jiang Z,Su C,Zheng C

Thu Dec 15 00:00:00 EST 2016

Herpes Simplex Virus 1 Tegument Protein UL41 Counteracts IFIT3 Antiviral Innate Immunity.

Journal of virology

Jiang Z,Su C,Zheng C

Thu Dec 15 00:00:00 EST 2016

Bioinformatics

Protein Aliases: GARG-39; Glucocorticoid-attenuated response gene 39 protein; IFI-54K; IFIT-2; Interferon, alpha-inducible protein (MW 54kD); Interferon-induced 54 kDa protein; interferon-induced protein 54; Interferon-induced protein with tetratricopeptide repeats 2; ISG-54 K

Gene Aliases: AV302338; CIG-42; cig42; G10P2; GARG-39; Garg39; IFI-54; IFI-54K; IFI54; IFIT-2; IFIT2; ISG-54 K; ISG-54K; ISG54; P54

UniProt ID: (Human) P09913, (Mouse) Q64112

Entrez Gene ID: (Human) 3433, (Mouse) 15958

Function(s)

apoptotic mitochondrial changes response to virus negative regulation of protein binding cellular response to interferon-alpha positive regulation of apoptotic process defense response to virus type I interferon signaling pathway immune system process apoptotic process innate immune response

Process(es)

RNA binding protein binding poly(A) RNA binding

IFIT2 Polyclonal Antibody

IFIT2 Antibody (12604-1-AP) in ICC/IF

Product Details

12604-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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