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|Tested species reactivity||Pig|
|Published species reactivity||Pig, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant porcine IFN gamma|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MP700 targets IFN gamma in ELISA, and WB applications and shows reactivity with Porcine samples.
The MP700 immunogen is recombinant porcine IFN gamma.
MP700 detects IFN gamma which has a predicted molecular weight of approximately 17 kDa.
The MP700 porcine IFNg antibody (clone PTF6) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MP701B (biotinylated conjugate of clone P2C11). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Time-course of antibody and cell-mediated immune responses to Porcine Reproductive and Respiratory Syndrome virus under field conditions.
MP700 was used in ELISA to follow antibody and cell-mediated immune responses to PRRSV infection in pigs living in a farm environment
|Dotti S,Guadagnini G,Salvini F,Razzuoli E,Ferrari M,Alborali GL,Amadori M||Research in veterinary science (94:510)||2013|
Co-incubation with IL-18 potentiates antigen-specific IFN-γ response in a whole-blood stimulation assay for measurement of cell-mediated immune responses in pigs experimentally infected with Lawsonia intracellularis.
MP700 was used in ELISA to study the effect of IL-18 on IFN gamma response in a whole-blood stimulation assay
|Riber U,Boesen HT,Jakobsen JT,Nguyen LT,Jungersen G||Veterinary immunology and immunopathology (139:257)||2011|
Characterization of the interferon-α response of pigs to the weaning stress.
MP700 was used in ELISA to investigate the characteristics of interferon alpha response in pigs with early weaning
|Razzuoli E,Villa R,Sossi E,Amadori M||Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research (31:237)||2011|
Immunogenicity and protective efficacy of an elastase-dependent live attenuated swine influenza virus vaccine administered intranasally in pigs.
MP700 was used in ELISA to characterize an elastase-dependent live attenuated influenza vaccine against swine influenza virus
|Masic A,Lu X,Li J,Mutwiri GK,Babiuk LA,Brown EG,Zhou Y||Vaccine (28:7098)||2010|
Elective cesarean delivery affects gut maturation and delays microbial colonization but does not increase necrotizing enterocolitis in preterm pigs.
MP700 was used in ELISA to study the effect of cesarean delivery on gut maturation and necrotizing enterocolitis
|Siggers RH,Thymann T,Jensen BB,Mølbak L,Heegaard PM,Schmidt M,Buddington RK,Sangild PT||American journal of physiology. Regulatory, integrative and comparative physiology (294:R929)||2008|
Efficacy and functionality of lipoprotein OprI from Pseudomonas aeruginosa as adjuvant for a subunit vaccine against classical swine fever.
MP700 was used in ELISA to study the role of OprI from P. aeruginosa against classical swine fever
|Rau H,Revets H,Cornelis P,Titzmann A,Ruggli N,McCullough KC,Summerfield A||Vaccine (24:4757)||2006|
Identification of classical swine fever virus protein E2 as a target for cytotoxic T cells by using mRNA-transfected antigen-presenting cells.
MP700 was used in ELISA to study the role of classical swine fever virus protein E2 in cytotoxic T cell effect
|Ceppi M,de Bruin MG,Seuberlich T,Balmelli C,Pascolo S,Ruggli N,Wienhold D,Tratschin JD,McCullough KC,Summerfield A||The Journal of general virology (86:2525)||2005|
Fibrocytes are potent stimulators of anti-virus cytotoxic T cells.
MP700 was used in ELISA to study the activation of anti-virus cytotoxic T cells
|Balmelli C,Ruggli N,McCullough K,Summerfield A||Journal of leukocyte biology (77:923)||2005|
Constitutive expression of interferons in swine leukocytes.
MP700 was used in immunoprecipitation to investigate the expression of interferons in the white blood cells of pigs
|Amadori M,Cristiano A,Ferrari M||Research in veterinary science (88:64)||2010|