|Tested species reactivity||Mouse|
|Published species reactivity||Fish, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified recombinant mouse IFN-gamma.|
|Storage buffer||whole serum diluted in PBS|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Key role for CD4 T cells during mixed antibody-mediated rejection of renal allografts.
AMC4034 was used in immunohistochemistry to study the role of CD4 T cells during mixed antibody-mediated rejection of renal allografts
|Gaughan A,Wang J,Pelletier RP,Nadasdy T,Brodsky S,Roy S,Lodder M,Bobek D,Mofatt-Bruce S,Fairchild RL,Henry ML,Hadley GA||American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons (14:284)||2014|
|Stochastic cytokine expression induces mixed T helper cell States.||Fang M,Xie H,Dougan SK,Ploegh H,van Oudenaarden A||PLoS biology (11:null)||2013|
Enhancement of antigen-presenting cell surface molecules involved in cognate interactions by immunostimulatory DNA sequences.
AMC4034 was used in blocking assay to demonstrate that immunostimulatory DNA up-regulates a distinct profile of cell surface molecules on macrophages and B cells in vitro and in vivo.
|Martin-Orozco E,Kobayashi H,Van Uden J,Nguyen MD,Kornbluth RS,Raz E||International immunology (11:1111)||1999|