|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 716 to 734 of human IKK alpha|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||20 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot applications, this antibody detects a band at ~85kDa. A suggested positive control is Hela cell lysate.
PA5-19897 can be used with blocking peptide PEP-0023.
Nuclear factor kappa B (NF-kappa-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. The long-sought Ikappa-B kinase (IKK), which phosphorylates Ikappa-B, and mediates Ikappa-B degradation and NF-kappa-B activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK-a and IKK-b). IKK-a and IKK-b interact with each other and both are essential for the NF-kappa-B activation. IKK-a specifically phosphorylates IkB-alpha. IKK-a is expressed in variety of human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.