In Western blot applications, this antibody detects a band at ~80kDa. A suggested positive control is Jurkat cell lysate.
PA5-19964 can be used with blocking peptide PEP-0089.
Nuclear factor kappa B (NF-kappa-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. Ikappa-B is phosphorylated by Ikappa-B kinase (IKK) complex that contains IKK-a, IKK-b, and IKK-gamma. A novel molecule in the IKK complex was recently identified and designated IKK-iota/IKK-i. IKK epsilon is required for the activation of NF-kappa-B by PMA and T cell receptors but not by TNF-a and IL-1. IKK-iota/IKK-i message is expressed in a variety of tissues and is inducible by TNF-a, IL-1, and LPS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: I-kappa-B kinase epsilon; IkBKE; IKK-E; IKK-epsilon; IKK-i; IKK-related kinase epsilon; Inducible I kappa-B kinase; inducible IkappaB kinase; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; RP11-534L20.1, IKK-E, IKK-i, IKKE, IKKI
Gene Aliases: IKBKE; IKK-E; IKK-i; IKKE; IKKI; KIAA0151
UniProt ID: (Human) Q14164
Entrez Gene ID: (Human) 9641