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Invitrogen

IL-10 Monoclonal Antibody (9D7)

24 References
View all (112) IL-10 antibodies

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Product Details

M010

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1000
-

ELISA (ELISA)

Assay-dependent
View 22 publications 22 publications

Inhibition Assays (Inhibition)

Assay-dependent
-

Miscellaneous PubMed (Misc)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

9D7

Immunogen

Recombinant human IL-10 (COS cell-derived)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Amount

500 µg

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223551

Product Specific Information

M010 targets IL-10 in ELISA, and IA applications and shows reactivity with Human samples.



The M010 immunogen is recombinant human IL-10 (COS cell-derived).



M010 detects IL-10 which has a predicted molecular weight of approximately 19 kDa.



The M010 IL10 antibody (clone 9D7) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M011B (biotinylated conjugate of clone 12G8). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.



Antibody M010 (clone 9D7) and biotinylated antibody M011B (clone 12G8) have successfully been used in combination with recombinant IL10 protein SIL10 in ELISA applications.



This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.



This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; IL10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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Function(s)
cytokine activity interleukin-10 receptor binding protein binding growth factor activity protein dimerization activity
Process(es)
negative regulation of cytokine production positive regulation of cytokine production positive regulation of endothelial cell proliferation response to molecule of bacterial origin chronic inflammatory response to antigenic stimulus positive regulation of immunoglobulin production negative regulation of cytokine production involved in immune response negative regulation of chronic inflammatory response to antigenic stimulus positive regulation of B cell apoptotic process immune response negative regulation of cell proliferation response to xenobiotic stimulus regulation of gene expression negative regulation of autophagy response to activity response to inactivity hemopoiesis B cell differentiation leukocyte chemotaxis negative regulation of myeloid dendritic cell activation negative regulation of B cell proliferation response to lipopolysaccharide negative regulation of interferon-alpha production negative regulation of interferon-gamma production negative regulation of interleukin-1 production negative regulation of interleukin-12 production negative regulation of interleukin-18 production negative regulation of interleukin-6 production negative regulation of interleukin-8 production negative regulation of tumor necrosis factor production response to insulin negative regulation of heterotypic cell-cell adhesion positive regulation of heterotypic cell-cell adhesion response to carbon monoxide cellular response to hepatocyte growth factor stimulus type 2 immune response B cell proliferation negative regulation of T cell proliferation defense response to bacterium defense response to protozoan positive regulation of macrophage activation negative regulation of apoptotic process negative regulation of neuron apoptotic process negative regulation of nitric oxide biosynthetic process regulation of isotype switching negative regulation of MHC class II biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of cell cycle positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade negative regulation of inflammatory response regulation of synapse organization negative regulation of membrane protein ectodomain proteolysis response to glucocorticoid negative regulation of cytokine activity branching involved in labyrinthine layer morphogenesis cellular response to lipopolysaccharide cellular response to estradiol stimulus negative regulation of chemokine (C-C motif) ligand 5 production endothelial cell apoptotic process liver regeneration interleukin-10-mediated signaling pathway positive regulation of plasma cell differentiation positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of response to wounding negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway regulation of complement-dependent cytotoxicity positive regulation of sprouting angiogenesis negative regulation of vascular smooth muscle cell proliferation positive regulation of vascular smooth muscle cell proliferation negative regulation of endothelial cell apoptotic process
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