|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids near the carboxy terminus of IL-1RAcP|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
In Western blot applications, this antibody detects a band at ~66kDa. A suggested positive control is Hela cell lysate.
PA5-19921 can be used with blocking peptide PEP-0047.
The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kappa-B inducing kinae (NIK) and of NF-kappa-B, recruites to the IL-1R complex through IL-1 RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Signal Transduction and Intracellular Trafficking by the Interleukin 36 Receptor.
PA5-19921 was used in western blot to elucidate the signaling pathway for interleukin 36 receptor
|Saha SS,Singh D,Raymond EL,Ganesan R,Caviness G,Grimaldi C,Woska JR,Mennerich D,Brown SE,Mbow ML,Kao CC||The Journal of biological chemistry (290:23997)||2015|