MM450DB targets IL-4 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM450DB immunogen is recombinant mouse IL-4.
MM450DB detects IL-4 which has a predicted molecular weight of approximately 14 kDa.
The MM450DB mouse IL4 antibody (biotinylated conjugate of clone 24G2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM450C (clone 1D11). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Biotinylated antibody MM450DB (clone 24G2) and antibody MM450C (clone 1D11) have successfully been used in combination with recombinant mouse IL4 protein SMIL4 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; IGG1 induction factor; IL-4; Interleukin-4; Lymphocyte stimulatory factor 1
Gene Aliases: BSF-1; Il-4; Il4
UniProt ID: (Mouse) P07750
Entrez Gene ID: (Mouse) 16189