|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||16 amino acid peptide near the carboxy terminus of human IL-36B|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||20 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A recommended positive control is A549 cell lysate.
At least two isoforms of IL-36B are known to exist; this antibody will only detect the longer isoform. IL-36B antibody will not cross-react with IL-36A or IL-36G.
IL-36B is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL-36B is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP. Like the related proteins IL-36A and IL-36G, IL-36B requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown. The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.