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Invitrogen
IL36B Polyclonal Antibody
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FIGURE: 1 / 1
IL36B Antibody (PA5-47066) in Neu
Neutralization of IL36B in human pre-adipocytes. Samples were incubated in IL36B polyclonal antibody (Product # PA5-47066). Recombinant Human IL‚36β/IL‚1F8 (aa 5-157) induces IL-8 secretion in human pre-adipocytes in a dose-dependent manner (orange line). IL-8 Secretion elicited by Recombinant Human IL‚36β/IL‚1F8 (aa 5-157) (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚36β/IL‚1F8 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 50‚300 ng/mL.
Product Details
PA5-47066
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human IL-36 beta/IL-1F8 Met1-Glu157
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) IL-1 beta and rhIL-1F6 is observed and less than 1% cross-reactivity with rhIL-1 alpha and rhIL-18 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IL-36B is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL-36B is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP. Like the related proteins IL-36A and IL-36G, IL-36B requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown. The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; hIL-1H2; family of interleukin 1-eta; FIL1 eta; IL-1 eta; IL-1F8; IL-1F8 (FIL1-eta); IL-1H2; Interleukin; interleukin 1 family, member 8 (eta); Interleukin-1 eta; Interleukin-1 family member 8; Interleukin-1 homolog 2; Interleukin-1 Superfamily e; Interleukin-36 beta; similar to IL-1
Gene Aliases: FIL1; FIL1-(ETA); FIL1H; FILI-(ETA); IL-1F8; IL-1H2; IL1-ETA; IL1F8; IL1H2; IL36B
UniProt ID: (Human) Q9NZH7
Entrez Gene ID: (Human) 27177
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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