|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to residues in human INPP5E|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 72 kDa.
Purity is >95% by SDS-PAGE.
INPP5E (inositol polyphosphate 5-phosphatase), also known as hosphatidylinositol polyphosphate 5-phosphatase type IV, is a 644 amino acid peripheral membrane protein associated with Golgi stacks. Belonging to the inositol-1,4,5-trisphosphate 5-phosphatase type IV family, INPP5E converts phosphatidylinositol-3,4,5-triphosphate (PtdIns 3,4,5-P3) to PtdIns-P2. While inactive towards water soluble inositol phosphates, the activity of INPP5E is specific for lipid substrates. INPP5E becomes phosphorylated upon DNA damage and is expressed in brain, heart, pancreas, testis and spleen. Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1), a disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. In addition, mutations in the INPP5E gene may lead to mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS), an autosomal recessive disorder characterized by moderate mental retardation, truncal obesity, congenital non-progressive retinal dystrophy, and micropenis in males.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.