Invitrogen

IQGAP1 Polyclonal Antibody

View all (24) IQGAP1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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IQGAP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of IQGAP1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IQGAP1 Polyclonal Antibody (Product # PA5-96385) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Alexa Fluor™ Plus 647 Phalloidin (Product # A30107, 1:2000 dilution). Panel d represents the merged image showing cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7A1110LZR) and externally deconvoluted (D.Sage et al. / Methods 115 (2017) 28–41).

IQGAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of IQGAP1 in paraffin-embedded human placenta using IQGAP1 Polyclonal Antibody (Product # PA5-96385) at a dilution of 1:200.

IQGAP1 Antibody in Western Blot (WB) — Western blot was performed using Anti-IQGAP1 Polyclonal Antibody (Product # PA5-96385) and a 189 kDa band corresponding to IQGAP1 was observed across all cell lysates tested. Whole cell extracts (60 µg lysate) of HaCaT (Lane 1), THP-1 (Lane 2), K-562 (Lane 3), HEK-293 (Lane 4) and A-431 (Lane 5) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). An uncharacterized band (*) was observed at ~60 kDa in HEK-293 and A-431 cell lysates.

Product Details

PA5-96385

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 500-750 of human IQGAP1. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.01% thimerosal
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2808187

Product Specific Information

Immunogen sequence: ELMKLKAQAH AENNEFITWN DIQACVDHVN LVVQEEHERI LAIGLINEAL DEGDAQKTLQ ALQIPAAKLE GVLAEVAQHY QDTLIRAKRE KAQEIQDESA VLWLDEIQGG IWQSNKDTQE AQKFALGIFA INEAVESGDV GKTLSALRSP DVGLYGVIPE CGETYHSDLA EAKKKKLAVG DNNSKWVKHW VKGGYYYYHN LETQEGGWDE PPNFVQNSMQ LSREEIQSSI SGVTAAYNRE QLWLANEGLI T; Positive Samples: A-549, 293T, HepG2, HT-29, HeLa; Cellular Location: Cell membrane

Target Information

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.