Invitrogen

IRAK-M Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (20) IRAK-M antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

IRAK-M Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Interleukin-1 receptor-associated kinase 3 was performed using 70% confluent log phase THP-1 and THP-1 differentiated with PMA (200 nM for 6 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IRAK-M Polyclonal Antibody (Product # PA5-89217) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma membrane and Cytoplasmic localization. Panel e represents THP-1 cells having no expression of IRAK-M. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

IRAK-M Antibody in Western Blot (WB) — Western blot was performed using Anti-IRAK-M Polyclonal Antibody (Product # PA5-89217) and a 60 kDa band corresponding to Interleukin-1 receptor-associated kinase 3 was observed in THP-1 cells differentiated with PMA and not in undifferentiated THP-1 cells. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1) and THP-1 treated with PMA (200 nM for 6 hours) (Lane 2) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).

IRAK-M Antibody in Western Blot (WB) — Western Blot analysis of IRAK-M in extracts of various cell lines using IRAK-M Polyclonal Antibody (Product # PA5-89217) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

IRAK-M Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of Interleukin-1 receptor-associated kinase 3 was observed in THP-1 cells differentiated with PMA and not in undifferentiated THP-1 cells using Anti-IRAK-M Polyclonal Antibody (Product # PA5-89217) in Western Blot. {RE}

IRAK-M Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-IRAK-M Polyclonal Antibody (Product # PA5-89217), shows expression of IRAK-M in THP-1 cells differentiated with PMA when compared to normal THP-1 cells. {RE}

Product Details

PA5-89217

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 367-596 of human IRAK3 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.88 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2805424

Product Specific Information

Immunogen sequence: VLTGCRVVLD DPKHIQLRDL LRELMEKRGL DSCLSFLDKK VPPCPRNFSA KLFCLAGRCA ATRAKLRPSM DEVLNTLEST QASLYFAEDP PTSLKSFRCP SPLFLENVPS IPVEDDESQN NNLLPSDEGL RIDRMTQKTP FECSQSEVMF LSLDKKPESK RNEEACNMPS SSCEESWFPK YIVPSQDLRP YKVNIDPSSE APGHSCRSRP VESSCSSKFS WDEYEQYKKE; Positive Samples: HL-60, THP-1, Mouse lung, Rat lung

Target Information

This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: FLJ13601; IL-1 receptor-associated kinase M; Interleukin-1 receptor-associated kinase 3; IRAK-3; IRAK-M; IRAK3

Gene Aliases: 4833428C18Rik; AI563835; ASRT5; IRAK-M; IRAK3; IRAKM

UniProt ID: (Human) Q9Y616, (Mouse) Q8K4B2

Entrez Gene ID: (Human) 11213, (Mouse) 73914, (Rat) 314870

negative regulation of cytokine-mediated signaling pathway MyD88-dependent toll-like receptor signaling pathway protein phosphorylation response to virus positive regulation of macrophage tolerance induction negative regulation of macrophage cytokine production cytokine-mediated signaling pathway negative regulation of NF-kappaB transcription factor activity response to peptidoglycan response to lipopolysaccharide negative regulation of interleukin-12 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production negative regulation of toll-like receptor signaling pathway negative regulation of protein catabolic process negative regulation of protein complex disassembly regulation of protein complex disassembly response to exogenous dsRNA negative regulation of MAP kinase activity negative regulation of innate immune response protein autophosphorylation positive regulation of NF-kappaB transcription factor activity interleukin-1-mediated signaling pathway response to interleukin-1 toll-like receptor signaling pathway apoptotic process signal transduction phosphorylation intracellular signal transduction regulation of apoptotic process biological_process

Process(es)

magnesium ion binding protein serine/threonine kinase activity ATP binding protein homodimerization activity protein heterodimerization activity nucleotide binding protein kinase activity protein binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups molecular_function