Invitrogen

IRAK1 Polyclonal Antibody

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6 Published Figures

View all (33) IRAK1 antibodies

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Questions & Answers

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Western Blot (WB)

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IRAK1 Antibody (PA5-17490) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), HT-1080 (Lane 4), COLO 205 (Lane 5), MDA-MB-231 (Lane 6), COS-7 (Lane 7) and THP-1 (Lane 8). The blot was probed with Anti-IRAK1 Polyclonal Antibody (Product # PA5-17490, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). An 80kDa band corresponding to IRAK1 was observed across all ... View More

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Immunocytochemistry (ICC/IF)

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Western Blot (WB)

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IRAK1 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), HT-1080 (Lane 4), COLO 205 (Lane 5), MDA-MB-231 (Lane 6), COS-7 (Lane 7) and THP-1 (Lane 8). The blot was probed with Anti-IRAK1 Polyclonal Antibody (Product # PA5-17490, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). An 80kDa band corresponding to IRAK1 was observed across all the cell lines tested.

IRAK1 Antibody in Western Blot (WB) — Western blot was performed using Anti-IRAK1 Polyclonal Antibody (Product # PA5-17490) and a 80 kDa band corresponding to Interleukin-1 receptor-associated kinase 1 was observed across tested cell lines. Whole cell extracts (40 µg lysate) of THP-1 (Lane 1), Jurkat (Lane 2), K-562 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

IRAK1 Antibody in Western Blot (WB) — Western blot analysis of IRAK1 in extracts from HeLa and 293 cells using IRAK1 polyclonal antibody (Product # PA5-17490).

IRAK1 Antibody in Western Blot (WB) — Knockdown of Interleukin-1 receptor-associated kinase 1 was achieved by transfecting K-562 with Interleukin-1 receptor-associated kinase 1 specific siRNAs (Silencer® select Product # s322, s324). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Interleukin-1 receptor-associated kinase 1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with IRAK1 Polyclonal Antibody (Product # PA5-17490, 1:1000 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Interleukin-1 receptor-associated kinase 1.

IRAK1 Antibody in Western Blot (WB) — Western blot analysis of IRAK1 in extracts from THP-1 cells, differentiated with TPA (80 nM for 24h) and treated with 1 µg/mL LPS, using IRAK1 polyclonal antibody (Product # PA5-17490).

IRAK1 Antibody in Immunocytochemistry (ICC/IF) — Published figure using IRAK1 polyclonal antibody (Product # PA5-17490) in Immunocytochemistry

IRAK1 Antibody in Western Blot (WB) — Published figure using IRAK1 polyclonal antibody (Product # PA5-17490) in Western Blot

IRAK1 Antibody in Western Blot (WB) — Published figure using IRAK1 polyclonal antibody (Product # PA5-17490) in Western Blot

IRAK1 Antibody in Western Blot (WB) — Published figure using IRAK1 polyclonal antibody (Product # PA5-17490) in Western Blot

IRAK1 Antibody in Western Blot (WB) — Fig 5 miR-UL112-3p inhibits TLR2-dependent activation of IRAK1. (A) Schematic of the upper section of the TLR2/NFkappaB pathway with known factors and post-translational modifications of IRAK4 and IRAK1 upon stimulation with TLR2 agonists. Grey ellipse, agonist; P, phosphorylation; K63, Lys63 poly-ubiquitin chain. (B) IRAK1 undergoes post-translational modifications upon stimulation with a TLR2/TLR1 agonist (PAM3CSK4, 100ng/ml) but not with a TLR4 agonist (LPS). TPA-differentiated THP-1 cells were treated as described in panel A. Unmodified and post-translationally modified forms of IRAK1 were detected by IB. (C) miR-UL112-3p inhibits FSL-1 induced IRAK1 post-translational modifications. TLR2, beta-actin and IRAK1 unmodified and modified forms were detected by IB. Numbers below the IRAK1 blot represent quantification (in relative units) of the unmodified protein signal.

IRAK1 Antibody in Western Blot (WB) — Published figure using IRAK1 polyclonal antibody (Product # PA5-17490) in Western Blot

IRAK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. K-562 cells were transfected with Interleukin-1 receptor-associated kinase 1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-IRAK1 Polyclonal Antibody (Product # PA5-17490). {KD}

Product Details

PA5-17490

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues surrounding glycine 696 of human IRAK1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

17 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10984291

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.

The Journal of biological chemistry

Dumbrepatil AB,Ghosh S,Zegalia KA,Malec PA,Hoff JD,Kennedy RT,Marsh ENG

Published figure using IRAK1 polyclonal antibody (Product # PA5-17490) in Western Blot

Fri Apr 26 00:00:00 UTC 2019

Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.

The Journal of biological chemistry

Dumbrepatil AB,Ghosh S,Zegalia KA,Malec PA,Hoff JD,Kennedy RT,Marsh ENG

Published figure using IRAK1 polyclonal antibody (Product # PA5-17490) in Western Blot

Fri Apr 26 00:00:00 UTC 2019

Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.

The Journal of biological chemistry

Dumbrepatil AB,Ghosh S,Zegalia KA,Malec PA,Hoff JD,Kennedy RT,Marsh ENG

Published figure using IRAK1 polyclonal antibody (Product # PA5-17490) in Western Blot

Fri Apr 26 00:00:00 UTC 2019

Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.

The Journal of biological chemistry

Dumbrepatil AB,Ghosh S,Zegalia KA,Malec PA,Hoff JD,Kennedy RT,Marsh ENG

Published figure using IRAK1 polyclonal antibody (Product # PA5-17490) in Western Blot

Fri Apr 26 00:00:00 UTC 2019

Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway.

PLoS pathogens

Landais I,Pelton C,Streblow D,DeFilippis V,McWeeney S,Nelson JA

Published figure using IRAK1 polyclonal antibody (Product # PA5-17490) in Western Blot

Fri May 01 00:00:00 UTC 2015

Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway.

PLoS pathogens

Landais I,Pelton C,Streblow D,DeFilippis V,McWeeney S,Nelson JA

Published figure using IRAK1 polyclonal antibody (Product # PA5-17490) in Western Blot

Fri May 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Interleukin-1 receptor-associated kinase 1; IRAK-1; Pelle homolog

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Gene Aliases: IRAK; IRAK1; pelle

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UniProt ID: (Human) P51617

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Entrez Gene ID: (Human) 3654

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protein kinase activity protein serine/threonine kinase activity NF-kappaB-inducing kinase activity protein binding ATP binding kinase activity heat shock protein binding protein homodimerization activity protein heterodimerization activity

activation of MAPK activity regulation of cytokine-mediated signaling pathway toll-like receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway protein phosphorylation signal transduction transmembrane receptor protein serine/threonine kinase signaling pathway activation of NF-kappaB-inducing kinase activity JNK cascade aging lipopolysaccharide-mediated signaling pathway negative regulation of NF-kappaB transcription factor activity positive regulation of type I interferon production response to lipopolysaccharide toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway toll-like receptor 9 signaling pathway cellular response to heat negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAP kinase activity innate immune response positive regulation of transcription, DNA-templated protein autophosphorylation positive regulation of smooth muscle cell proliferation positive regulation of NF-kappaB transcription factor activity protein oligomerization nucleotide-binding oligomerization domain containing signaling pathway interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to hypoxia

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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