Invitrogen

IRAK1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 Published Figures

3 References

View all (33) IRAK1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

IRAK1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of IRAK1 in HT-1080 cells using an IRAK1 polyclonal antibody (Product # PA5-17490) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

IRAK1 Antibody in Western Blot (WB) — Western blot was performed using Anti-IRAK1 Polyclonal Antibody (Product # PA5-17490) and a 80 kDa band corresponding to Interleukin-1 receptor-associated kinase 1 was observed across tested cell lines. Whole cell extracts (40 µg lysate) of THP-1 (Lane 1), Jurkat (Lane 2), K-562 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

IRAK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. K-562 cells were transfected with Interleukin-1 receptor-associated kinase 1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-IRAK1 Polyclonal Antibody (Product # PA5-17490).

Product Details

PA5-17490

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues surrounding glycine 696 of human IRAK1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

26.4 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

RRID

AB_10984291

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Interleukin-1 receptor-associated kinase 1 (IRAK1) is a serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. IRAK is associated with Toll-like receptor (TLR) and IL-1R signaling pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.

The Journal of biological chemistry

Dumbrepatil AB,Ghosh S,Zegalia KA,Malec PA,Hoff JD,Kennedy RT,Marsh ENG

PA5-17490 was used in Immunocytochemistry-immunoflourescence to conclude that the synergistic activation of viperin and IRAK1 provides a mechanism that couples innate immune signaling with the production of the antiviral nucleotide ddhCTP.

Fri Apr 26 00:00:00 EDT 2019

Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.

The Journal of biological chemistry

Dumbrepatil AB,Ghosh S,Zegalia KA,Malec PA,Hoff JD,Kennedy RT,Marsh ENG

Fri Apr 26 00:00:00 EDT 2019

Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.

The Journal of biological chemistry

Dumbrepatil AB,Ghosh S,Zegalia KA,Malec PA,Hoff JD,Kennedy RT,Marsh ENG

Fri Apr 26 00:00:00 EDT 2019

Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.

The Journal of biological chemistry

Dumbrepatil AB,Ghosh S,Zegalia KA,Malec PA,Hoff JD,Kennedy RT,Marsh ENG

Fri Apr 26 00:00:00 EDT 2019

Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway.

PLoS pathogens

Landais I,Pelton C,Streblow D,DeFilippis V,McWeeney S,Nelson JA

PA5-17490 was used in western blot to study the effect of human cytomegalovirus microRNAs on TLR2.

Fri May 01 00:00:00 EDT 2015

Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway.

PLoS pathogens

Landais I,Pelton C,Streblow D,DeFilippis V,McWeeney S,Nelson JA

PA5-17490 was used in western blot to study the effect of human cytomegalovirus microRNAs on TLR2.

Fri May 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Interleukin-1 receptor-associated kinase 1; IRAK-1; Pelle homolog

Gene Aliases: IRAK; IRAK1; pelle

UniProt ID: (Human) P51617

Entrez Gene ID: (Human) 3654

Function(s)

activation of MAPK activity regulation of cytokine-mediated signaling pathway toll-like receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway protein phosphorylation signal transduction transmembrane receptor protein serine/threonine kinase signaling pathway activation of NF-kappaB-inducing kinase activity JNK cascade aging lipopolysaccharide-mediated signaling pathway negative regulation of NF-kappaB transcription factor activity positive regulation of type I interferon production response to lipopolysaccharide toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway toll-like receptor 9 signaling pathway cellular response to heat negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAP kinase activity innate immune response positive regulation of transcription, DNA-templated protein autophosphorylation positive regulation of smooth muscle cell proliferation positive regulation of NF-kappaB transcription factor activity protein oligomerization nucleotide-binding oligomerization domain containing signaling pathway interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to hypoxia

Process(es)

protein kinase activity protein serine/threonine kinase activity NF-kappaB-inducing kinase activity protein binding ATP binding kinase activity heat shock protein binding protein homodimerization activity protein heterodimerization activity

IRAK1 Polyclonal Antibody

Product Details

PA5-17490

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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