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Invitrogen
IRF7 Recombinant Rat Monoclonal Antibody (RDP4ND4)
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FIGURE: 1 / 6
IRF7 Antibody (740138T) in ICC/IF
Immunofluorescence analysis of IRF7 was performed using IRF7 Recombinant Rat Monoclonal Antibody (RDP4ND4) (Product # 740138T) on 70-80% confluent PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with IRF7 Recombinant Rat Monoclonal Antibody (RDP4ND4) (Product #740138T, 1:200) in 0.1% BSA, incubated at 4 degree Celsius overnight, followed by labelling with Donkey anti-Rat IgG (H+L) High... View More
Product Details
740138T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
RDP4ND4
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Interferon regulatory factor 7; interferon regulatory factor-7H; IRF-7
Gene Aliases: IMD39; IRF-7H; IRF7; IRF7A; IRF7B; IRF7C; IRF7H
UniProt ID: (Human) Q92985
Entrez Gene ID: (Human) 3665
negative regulation of transcription from RNA polymerase II promoter
regulation of adaptive immune response
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
response to virus
immunoglobulin mediated immune response
establishment of viral latency
regulation of type I interferon production
positive regulation of type I interferon production
interferon-alpha production
interferon-beta production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
regulation of MyD88-dependent toll-like receptor signaling pathway
regulation of MyD88-independent toll-like receptor signaling pathway
TRIF-dependent toll-like receptor signaling pathway
MDA-5 signaling pathway
innate immune response
type I interferon biosynthetic process
regulation of monocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of immune response
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
positive regulation of type I interferon-mediated signaling pathway
negative regulation of macrophage apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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