PA1-1058 detects a IRS-1 in mouse and human cells.
PA1-1058 has been successfully used in Western blot procedures. By Western blot this antibody detects an ~170 kDa protein representing IRS-1 in 3T3 L1 cell extract.
The PA1-1058 immunogen is a synthetic peptide corresponding to residues L(602) H T D D G Y M P M S P G V(615) of human IRS-1. The immunizing peptide is 92% conserved in rats, and mice. This peptide (Cat. # PEP-192) is available for use in neutralization and control experiments.
Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS-1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS-1 degradation pathways are not well understood. IRS-1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS-1 phosphorylation and subsequent degradation an attractive therapeutic option.To date there have been four subtypes identified: IRS-1,2,3, and 4, with IRS-1 being widely expressed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Insulin receptor substrate 1; Insulin Receptor Substrate-1; IRS-1
Gene Aliases: G972R; HIRS-1; IRS-1; IRS1