Proteintech

IST1 Polyclonal Antibody

Product Image Gallery

IST1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using KIAA0174 antibody 51002-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

IST1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 51002-1-AP (IST1 antibody) at dilution of 1:200 (under 10x lens).

IST1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 51002-1-AP (IST1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Product Details

51002-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:8,000	View 6 publications 6 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 7 publications 7 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IST1 Fusion Protein Ag0721 (1-364 aa encoded by BC000430) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MLGSGFKAE RLRVNLRLVI NRLKLLEKKK TELAQKARKE IADYLAAGKD ERARIRVEHI IREDYLVEAM EILELYCDLL LARFGLIQSM KELDSGLAES VSTLIWAAPR LQSEVAELKI VADQLCAKYS KEYGKLCRTN QIGTVNDRLM HKLSVEAPPK ILVERYLIEI AKNYNVPYEP DSVVMAEAPP GVETDLIDVG FTDDVKKGGP GRGGSGGFTA PVGGPDGTVP MPMPMPMPMP SANTPFSYPL PKGPSDFNGL PMGTYQAFPN IHPPQIPATP PSYESMTLML IRISLLHRLL VLDPSQKPLQ SFLPDLQITM TTLSYQSCHL CQTHYQLHLL VPAPQHLKTL TLMIFPGGLK S (1-364 aa encoded by BC000430)

Target Information

Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Nature

Liu S,Kwon M,Mannino M,Yang N,Renda F,Khodjakov A,Pellman D

Sat Sep 01 00:00:00 EDT 2018

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell reports

Goliand I,Adar-Levor S,Segal I,Nachmias D,Dadosh T,Kozlov MM,Elia N

Tue Aug 14 00:00:00 EDT 2018

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Scientific reports

Ott C,Nachmias D,Adar S,Jarnik M,Sherman S,Birnbaum RY,Lippincott-Schwartz J,Elia N

Tue Feb 20 00:00:00 EST 2018

An Automated Image Analysis System to Quantify Endosomal Tubulation.

PloS one

Newton TM,Reid E

Tue Jul 18 00:00:00 EDT 2017

Defects in ER-endosome contacts impact lysosome function in hereditary spastic paraplegia.

The Journal of cell biology

Allison R,Edgar JR,Pearson G,Rizo T,Newton T,Günther S,Berner F,Hague J,Connell JW,Winkler J,Lippincott-Schwartz J,Beetz C,Winner B,Reid E

Mon May 01 00:00:00 EDT 2017

ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission.

The Journal of cell biology

Christ L,Wenzel EM,Liestøl K,Raiborg C,Campsteijn C,Stenmark H

Mon Feb 29 00:00:00 EST 2016

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Nature

Vietri M,Schink KO,Campsteijn C,Wegner CS,Schultz SW,Christ L,Thoresen SB,Brech A,Raiborg C,Stenmark H

Thu Jun 11 00:00:00 EDT 2015

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Nature

Vietri M,Schink KO,Campsteijn C,Wegner CS,Schultz SW,Christ L,Thoresen SB,Brech A,Raiborg C,Stenmark H

Thu Jun 11 00:00:00 EDT 2015

An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome.

The Journal of cell biology

Allison R,Lumb JH,Fassier C,Connell JW,Ten Martin D,Seaman MN,Hazan J,Reid E

Mon Aug 05 00:00:00 EDT 2013

Over-expression of the overexpressed in lung cancer 1 is associated with poor prognosis in epithelial ovarian cancer.

Journal of surgical oncology

Jia C,Li X,Sun H,Sui L

Sat Jun 01 00:00:00 EDT 2013

Over-expression of the overexpressed in lung cancer 1 is associated with poor prognosis in epithelial ovarian cancer.

Journal of surgical oncology

Jia C,Li X,Sun H,Sui L

Sat Jun 01 00:00:00 EDT 2013

ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes.

Proceedings of the National Academy of Sciences of the United States of America

Hadders MA,Agromayor M,Obita T,Perisic O,Caballe A,Kloc M,Lamers MH,Williams RL,Martin-Serrano J

Tue Oct 23 00:00:00 EDT 2012

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.

Structure (London, England : 1993)

Agromayor M,Soler N,Caballe A,Kueck T,Freund SM,Allen MD,Bycroft M,Perisic O,Ye Y,McDonald B,Scheel H,Hofmann K,Neil SJ,Martin-Serrano J,Williams RL

Wed Mar 07 00:00:00 EST 2012

Essential role of hIST1 in cytokinesis.

Molecular biology of the cell

Agromayor M,Carlton JG,Phelan JP,Matthews DR,Carlin LM,Ameer-Beg S,Bowers K,Martin-Serrano J

Sun Mar 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: hIST1; increased sodium tolerance 1 homolog; IST1 homolog; IST1, endosomal sorting complex required for transport-III component; MGC117220; overexpressed in lung cancer 1; pm28; Putative MAPK-activating protein PM28

Gene Aliases: IST1; KIAA0174; OLC1

UniProt ID: (Human) P53990

Entrez Gene ID: (Human) 9798

Function(s)