Immunofluorescent analysis of Influenza A PB2 in A/WSN/33 infected Vero cells. A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min and stained using an Influenza A PB2 polyclonal antibody (Product # PA5-32221) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.
|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment of influenza A virus PB2 (A/Puerto Rico/8/1934(H1N1))|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32221 targets Anti-Influenza A Virus PB2 in WB applications and shows reactivity with Virus samples.
The PA5-32221 immunogen is recombinant protein fragment of influenza A virus PB2 (A/Puerto Rico/8/1934(H1N1)).
Influenza A virus contains eight single stranded, negative sense RNAs. Each RNA is associated with viral RNA-dependent RNA polymerase which consists of PA, PB1 and PB2 proteins. PB1 is the subunit involved in the catalytic activity of nucleotide polymerization and is involved in the initiation of transcription. Both PB1 and PB2 can be crosslinked to synthetic RNA with the 3' terminal sequence of viral RNA. PB1 and PB2 may also be involved in recognition of the promoter and/or replication origin on template viral RNA. PB2 is the cap 1-recognition protein, which binds to the cap structure of host mRNA. The cap structure then acts as a primer for transcription to produce viral mRNA.
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