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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Insulin Antibodies

          Bioss

          Insulin Polyclonal Antibody

          View all (61) Insulin antibodies

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          Cite Insulin Polyclonal Antibody

          • Antibody Testing Data (1)
          Insulin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Insulin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Insulin Antibody (BS-0862R) in WB

          Lane 1: human islet alpha lysates Lane 2: human islet beta lysates probed with Rabbit Anti-Insulin Polyclonal Antibody, Unconjugated (bs-0862R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Insulin Antibody in Western Blot (WB)
          Insulin Polyclonal Antibody

          Product Details

          BS-0862R

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:300
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          -

          ELISA (ELISA)

          1:500-1:1,000
          -

          Immunoprecipitation (IP)

          1-2 µg
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Pig, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH conjugated Insulin from porcine pancreas.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

          Contains

          0.02% ProClin 300

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Ins; Insulin; Insulin-1; Insulin-2; preproinsulin; proinsulin

          View more View less

          Gene Aliases: AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins-1; Ins-2; Ins1; Ins2; Ins2-rs1; InsII; IRDN; Mody; MODY10; Mody4; proinsulin

          View more View less

          UniProt ID: (Pig) P01315, (Human) P01308, (Rat) P01322, (Mouse) P01325, (Rat) P01323, (Mouse) P01326

          View more View less

          Entrez Gene ID: (Pig) 397415, (Human) 3630, (Rat) 24505, (Mouse) 16333, (Rat) 24506, (Mouse) 16334

          View more View less

          Function(s)
          protease binding insulin receptor binding insulin-like growth factor receptor binding hormone activity identical protein binding protein binding chaperone binding protein complex binding growth factor
          Process(es)
          negative regulation of acute inflammatory response glucose metabolic process regulation of cellular amino acid metabolic process acute-phase response G-protein coupled receptor signaling pathway positive regulation of cell proliferation insulin receptor signaling pathway lipid biosynthetic process glycoprotein biosynthetic process positive regulation of nitric oxide mediated signal transduction positive regulation of glucose metabolic process positive regulation of phosphatidylinositol 3-kinase signaling lactate biosynthetic process regulation of transmembrane transporter activity positive regulation of cell migration positive regulation of protein autophosphorylation activation of protein kinase B activity negative regulation of NAD(P)H oxidase activity wound healing lipoprotein biosynthetic process negative regulation of protein catabolic process glucose homeostasis positive regulation of MAPK cascade negative regulation of gluconeogenesis positive regulation of fatty acid biosynthetic process positive regulation of glycogen biosynthetic process positive regulation of DNA replication negative regulation of glycogen catabolic process positive regulation of glycolytic process positive regulation of mitotic nuclear division negative regulation of proteolysis negative regulation of fatty acid metabolic process positive regulation of glucose import positive regulation of insulin receptor signaling pathway alpha-beta T cell activation negative regulation of protein secretion positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipid catabolic process positive regulation of lipoprotein lipase activity positive regulation of NF-kappaB transcription factor activity positive regulation of protein kinase B signaling fatty acid homeostasis negative regulation of respiratory burst involved in inflammatory response positive regulation of respiratory burst positive regulation of protein localization to nucleus positive regulation of dendritic spine maintenance regulation of protein localization to plasma membrane negative regulation of reactive oxygen species biosynthetic process response to L-arginine neuron projection maintenance negative regulation of feeding behavior MAPK cascade regulation of transcription, DNA-templated ER to Golgi vesicle-mediated transport cell-cell signaling positive regulation of gene expression glucose transport positive regulation of cell growth positive regulation of cellular protein metabolic process negative regulation of protein oligomerization regulation of protein localization cellular protein metabolic process positive regulation of nitric oxide biosynthetic process positive regulation of cell differentiation negative regulation of vasodilation positive regulation of vasodilation positive regulation of lipid biosynthetic process regulation of protein secretion positive regulation of nitric-oxide synthase activity positive regulation of peptide hormone secretion positive regulation of brown fat cell differentiation negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway response to organic substance response to cytokine response to peptide hormone response to cAMP carbohydrate metabolic process negative regulation of transcription from RNA polymerase II promoter activation of MAPK activity ER overload response myoblast fusion biological_process lipid catabolic process regulation of protein binding positive regulation of phosphatidylinositol 3-kinase activity canonical Wnt signaling pathway intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress activation of JUN kinase activity regulation of gene expression myotube differentiation regulation of phosphorylation
          It has to be done as per old AB suggested Products section.
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