|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an internal region of the human JMJD6 protein (within residues 150-350).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.1% BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The target sequence has 98% sequence homology with mouse, rat and bovine, and 91% sequence homology with xenopus and zebrafish.
Jumonji domain containing 6 is a histone demethylating enzyme, which is active on arginine residues, but not lysines. Its activity destabilizes heterochromatin structures and activates gene expressions in euchromatin. In endothelial cells, the activity of JMJD6 is necessary for angiogenesis. If JMJD6 expression is reduced, the VEGF-receptor (Flt-1) is incorrectly spliced, leading to increased soluble Flt-1 which binds VEGF and PlGF and inhibits angiogenesis. JMJD6 also hydroxylates RNA splicing factors, and is important in embryonic differentiation. It most likely interacts specifically with single-stranded RNA, which may contribute to its early differentiation importance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.