Invitrogen

Jagged1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (22) Jagged1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Jagged1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Jagged1 in immersion fixed paraffin-embedded sections of human kidney cancer tissue. Samples were incubated in Jagged1 polyclonal antibody (Product # PA5-46970) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).

Jagged1 Antibody in Western Blot (WB) — Western blot analysis of Jagged1 in Huh‚7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. Samples were incubated in Jagged1 polyclonal antibody (Product # PA5-46970) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions.

Jagged1 Antibody in Neutralization (Neu) — Neutralization of Jagged1 in C3H10T1/2 mouse embryonic fibroblast cell line. Samples were incubated in Jagged1 polyclonal antibody (Product # PA5-46970). Recombinant Human Jagged 1 Fc Chimera induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Jagged 1 Antibody. The ND50 is typically 1-5 µg/mL.

Jagged1 Antibody — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of Jagged1 is shown by the decrease in mean OD (measure of alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cell line) with increasing concentrations of Jagged1 Polyclonal Antibody (PA5-46970). {Neu}

Product Details

PA5-46970

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	5-15 µg/mL	-
Neutralization (Neu)	1-5 µg/mL	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Jagged 1 Ser32-Asp296 View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2608268

Product Specific Information

In direct ELISAs, approximately 20% cross-reactivity with recombinant rat Jagged 1 is observed and less than 1% cross-reactivity with recombinant human Jagged 2 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Notch1 signaling determines the plasticity and function of fibroblasts in diabetic wounds.

Life science alliance

Shao H,Li Y,Pastar I,Xiao M,Prokupets R,Liu S,Yu K,Vazquez-Padron RI,Tomic-Canic M,Velazquez OC,Liu ZJ

PA5-46970 was used in Western Blotting to show that Notch1 signaling is a molecular determinant controlling the plasticity and function of fibroblasts by modulating wound healing and angiogenesis.

Tue Dec 01 00:00:00 EST 2020

Notch1 signaling determines the plasticity and function of fibroblasts in diabetic wounds.

Life science alliance

Shao H,Li Y,Pastar I,Xiao M,Prokupets R,Liu S,Yu K,Vazquez-Padron RI,Tomic-Canic M,Velazquez OC,Liu ZJ

PA5-46970 was used in Western Blotting to show that Notch1 signaling is a molecular determinant controlling the plasticity and function of fibroblasts by modulating wound healing and angiogenesis.

Tue Dec 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: CD339; jagged canonical Notch ligand 1; Jagged1; Protein jagged-1

Gene Aliases: AGS; AHD; AWS; CD339; HJ1; JAG1; JAGL1

UniProt ID: (Human) P78504

Entrez Gene ID: (Human) 182

Function(s)

angiogenesis cell fate determination blood vessel remodeling morphogenesis of an epithelial sheet T cell mediated immunity pulmonary valve morphogenesis cardiac right ventricle morphogenesis Notch signaling pathway Notch receptor processing nervous system development hemopoiesis keratinocyte differentiation regulation of cell migration response to muramyl dipeptide aorta morphogenesis regulation of cell proliferation auditory receptor cell differentiation myoblast differentiation endothelial cell differentiation negative regulation of fat cell differentiation positive regulation of myeloid cell differentiation negative regulation of neuron differentiation positive regulation of osteoblast differentiation positive regulation of Notch signaling pathway positive regulation of transcription from RNA polymerase II promoter cardiac septum morphogenesis ciliary body morphogenesis pulmonary artery morphogenesis cardiac neural crest cell development involved in outflow tract morphogenesis Notch signaling involved in heart development endocardial cushion cell development nephron development glomerular visceral epithelial cell development distal tubule development loop of Henle development neuronal stem cell population maintenance negative regulation of stem cell differentiation

Process(es)

Notch binding structural molecule activity calcium ion binding protein binding growth factor activity

Jagged1 Polyclonal Antibody

Product Details

PA5-46970

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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