Invitrogen

KCNK6 Polyclonal Antibody

View all (8) KCNK6 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (5)

FIGURE: 1 / 5

KCNK6 Antibody (PA5-119866) in WB

Western blot analysis of KCNK6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. KCNK6 Polyclonal Antibody (Product # PA5-119866) at 1:1,000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate. Lane 2: Rat ovary tissue lysate.

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KCNK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse small intestine tissue using KCNK6 Polyclonal Antibody (Product # PA5-119866). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the KCNK6 antibody at a dilution of 1:100 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Details

PA5-119866

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within C-terminal human KCNK6 from aa 291-aa313

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2913438

Product Specific Information

Positive Control: Jurkat cell lysate, rat ovary tissue lysate, human kidney tissue, human placenta tissue, mouse small intestine tissue, rat brain tissue.

Subcellular Location: Membrane.

Target Information

This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. This channel protein, considered an open rectifier, is widely expressed. It is stimulated by arachidonic acid, and inhibited by internal acidification and volatile anaesthetics. [provided by RefSeq, Jul 2008].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ12282; Inward rectifying potassium channel protein TWIK-2; K2P6.1 potassium channel; Potassium channel subfamily K member 6; potassium channel, subfamily K, member 6; potassium channel, two pore domain subfamily K, member 6; potassium inwardly-rectifying channel, subfamily K, member 6; TWIK-2 two-pore-domain K+ channel; TWIK-originated similarity sequence; TWIK-originated sodium similarity sequence

Gene Aliases: D7Ertd764e; K2p6.1; KCNK6; KCNK8; TOSS; TWIK-2; TWIK2

UniProt ID: (Human) Q9Y257

Entrez Gene ID: (Human) 9424, (Rat) 116491, (Mouse) 52150

Molecular Function: ion channel transporter