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KDEL Receptor Pan Antibody (PA5-101011) in ICC/IF
Immunofluorescent analysis of KDEL Receptor Pan in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and KDEL Receptor Pan polyclonal antibody (Product # PA5-101011) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG... View More
Product Details
PA5-101011
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KDELR1(Accession P24390), corresponding to amino acid residues N89-S139.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850487
Product Specific Information
Antibody detects endogenous levels of total KDEL.
Target Information
Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 2; ELP-1; ER lumen protein retaining receptor 1; ER lumen protein retaining receptor 2; ER lumen protein retaining receptor 3; ER lumen protein-retaining receptor 1; ER lumen protein-retaining receptor 2; ER lumen protein-retaining receptor 3; ERD-2-like protein; ERD2-like protein 1; ERD23; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 2; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3; KDEL endoplasmic reticulum protein retention receptor 1; KDEL endoplasmic reticulum protein retention receptor 2; KDEL endoplasmic reticulum protein retention receptor 3; KDEL receptor 1; KDEL receptor 2; KDEL receptor 3; Putative MAPK-activating protein PM23
Gene Aliases: 1110007A14Rik; 8030486F04Rik; AI173274; AW215843; ELP-1; ERD2; ERD2.1; ERD2.2; ERD23; ERD2L3; HDEL; KDELR1; KDELR2; KDELR3; PM23
UniProt ID: (Human) P24390, (Human) P33947, (Human) O43731, (Mouse) Q99JH8, (Rat) Q569A6, (Mouse) Q9CQM2, (Rat) Q5U305, (Mouse) Q8R1L4
Entrez Gene ID: (Human) 10945, (Human) 11014, (Human) 11015, (Mouse) 68137, (Rat) 361577, (Mouse) 66913, (Rat) 304290, (Rat) 315131, (Mouse) 105785
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