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KGF Antibody (500-P19-100UG) in WB
Western Blot: To detect Human KGF (FGF-7) by Western Blot analysis KGF Polyclonal Antibody (Product # 500-P19-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human KGF (FGF-7) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P19-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human KGF (FGF-7)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929747
Product Specific Information
AA Sequence of recombinant protein: CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human KGF (FGF-7). Anti-Human KGF (FGF-7)-specific antibody was purified by affinity chromatography employing an immobilized Human KGF (FGF-7) matrix.
Sandwich ELISA: To detect Human KGF (FGF-7) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human KGF (FGF-7) (500-P19Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human KGF (FGF-7).
Western Blot: To detect Human KGF (FGF-7) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human KGF (FGF-7) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FGF-7; Fibroblast growth factor 7; HBGF-7; Heparin-binding growth factor 7; Keratinocyte growth factor
Gene Aliases: FGF7; HBGF-7; KGF
UniProt ID: (Human) P21781
Entrez Gene ID: (Human) 2252
protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
type 2 fibroblast growth factor receptor binding
protein binding
growth factor activity
heparin binding
1-phosphatidylinositol-3-kinase activity
chemoattractant activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
growth factor
intercellular signal molecule
MAPK cascade
ovarian follicle development
positive regulation of defense response to virus by host
phosphatidylcholine biosynthetic process
signal transduction
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
epidermis development
response to wounding
mesenchymal cell proliferation
positive regulation of keratinocyte proliferation
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
hair follicle morphogenesis
actin cytoskeleton reorganization
protein localization to cell surface
phosphatidylinositol-3-phosphate biosynthetic process
wound healing
surfactant homeostasis
positive regulation of GTPase activity
positive regulation of transcription, DNA-templated
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of epithelial cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
positive chemotaxis
positive regulation of keratinocyte migration
positive regulation of cell division
branching involved in salivary gland morphogenesis
positive regulation of epithelial cell proliferation involved in lung morphogenesis
regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling
secretion by lung epithelial cell involved in lung growth
xenophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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