Invitrogen

KIDINS220 Polyclonal Antibody

View all (15) KIDINS220 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

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KIDINS220 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of KIDINS220 in PC-12 cells using a KIDINS220 polyclonal antibody (Product #PA5-116475). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

KIDINS220 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of KIDINS220 in a paraffin-embedded human breast cancer tissue using a KIDINS220 polyclonal antibody (Product #PA5-116475). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

KIDINS220 Antibody in Western Blot (WB) — Western blot analysis of KIDINS220 in SH-SY5Y cell lysate using a KIDINS220 polyclonal antibody (Product #PA5-116475). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

KIDINS220 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of KIDINS220 in SH-SY5Y cells using a KIDINS220 polyclonal antibody (Product#PA5-116475). The cells were fixed, permeabilize, and stained with the primary antibody (1:50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1000 dilution for 30 minutes. The control was not incubated with the primary antibody (black).

Product Details

PA5-116475

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide within Human Kidins220 aa 1,325-1,374 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2901107

Product Specific Information

Positive controls: SH-SY5Y cell lysates, N2A, PC-12, human pancreas tissue, human breast cancer tissue, mouse brain tissue, SH-SY5Y.

Target Information

This gene encodes a transmembrane protein that is preferentially expressed in the nervous system where it controls neuronal cell survival, differentiation into exons and dendrites, and synaptic plasticity. The encoded protein interacts with membrane receptors, cytosolic signaling components, and cytoskeletal proteins, serving as a scaffold that mediates crosstalk between the neurotrophin pathway and several other intracellular signaling pathways. Aberrant expression of this gene is associated with the onset of various neuropsychiatric disorders and neurodegenerative diseases, including Alzheimer's disease. Naturally occurring mutations in this gene are associated with a syndrome characterized by spastic paraplegia, intellectual disability, nystagmus and obesity. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ankyrin repeat-rich membrane spanning; ankyrin repeat-rich membrane spanning protein; Ankyrin repeat-rich membrane-spanning protein; kinase D-interacting substance 220; kinase D-interacting substance of 220 kDa; Kinase D-interacting substrate of 220 kDa; kinase D-interacting substrate, 220kDa; MGC163482; truncated KIDINS220

Gene Aliases: 3110039L19Rik; AI194387; AI316525; ARMS; C330002I19Rik; KIAA1250; KIDINS220; mKIAA1250

UniProt ID: (Human) Q9ULH0, (Rat) Q9ERD4

Entrez Gene ID: (Human) 57498, (Rat) 116478, (Mouse) 77480

Function(s)

in utero embryonic development positive regulation of neuron projection development cytosolic transport nerve growth factor signaling pathway regulation of protein kinase activity dendrite morphogenesis cellular response to nerve growth factor stimulus activation of MAPKK activity

Process(es)

protein kinase regulator activity PDZ domain binding protein binding protein kinase binding

KIDINS220 Polyclonal Antibody

Product Details

PA5-116475

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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