Invitrogen

KIF3A Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (12) KIF3A antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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KIF3A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of KIF3A in mouse brain tissue using KIF3A Polyclonal Antibody (Product # PA1-20240) at a dilution of 0.4 µg/mL.

KIF3A Antibody in Western Blot (WB) — Western blot was performed using Anti-KIF3A Polyclonal Antibody (Product # PA1-20240) and a 80 kDa band corresponding to KIF3A was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of LNCaP (Lane 1), PC-3 (Lane 2), Jurkat (Lane 3), MOLT-4 (Lane 4), SH-SY5Y (Lane 5), U-2 OS (Lane 6), SK-OV-3 (Lane 7), A-431 (Lane 8) and tissue extracts (30 µg lysate) of Mouse Brain (Lane 9) and Mouse Testis (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

KIF3A Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. LNCaP cells were transfected with KIF3A siRNA and reduction of signal was observed in Western Blot using KIF3A Polyclonal Antibody (Product # PA1-20240). {KD}

Product Details

PA1-20240

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide, KLH conjugated, corresponding to residues 682-701 of mouse KIF3A. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	5.3 mg/mL
Purification	IgG fraction
Storage buffer	0.01M PBS, pH 7.4
Contains	15mM sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_559814

Product Specific Information

Recommended positive controls: PC12, rat cerebellum.

Target Information

The kinesins constitute a large family of microtubule-dependent motor proteins which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events including endocytosis and transcytosis. Members of the heterotrimeric kinesin II family of microtubule associated motors generally contain two different motor subunits from the KIF3 family, which includes KIF3A, B and C. KIF3 isoforms mediate anterograde transport of membrane bound organelles in neurons and melanosomes, transport between the endoplasmic reticulum and the Golgi, and transport of protein complexes within cilia and flagella required for their morphogenesis. KIF3A may influence neurogenesis at the level of embryonic cellular events, where the asymmetry of the genetic control circuit controlling left-right (L-R) axis determination is defined. Loss of KIF3A function in mice photoreceptors causes apoptotic cell death, suggesting that kinesin II mediated transport is required for proper cell fate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

KIF1-binding protein interacts with KIF3A in haploid male germ cells.

Reproduction (Cambridge, England)

Lehti MS,Kotaja N,Sironen A

PA1-20240 was used in immunohistochemistry and western blot to investigate the interaction between KIF1-binding protein and KIF3A in haploid male germ cells

Tue Sep 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: kinesin family protein 3A; kinesin-II subunit; Kinesin-like protein KIF3A; Microtubule plus end-directed kinesin motor 3A; N-4 kinesin

Gene Aliases: FLA10; KIF3; KIF3A; Kifl; KLP-20; Kns3

UniProt ID: (Human) Q9Y496, (Mouse) P28741

Entrez Gene ID: (Human) 11127, (Mouse) 16568, (Rat) 84392

Molecular Function: microtubule binding motor protein