Invitrogen

KMO Polyclonal Antibody

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KMO Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded KMO in mouse liver tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with KMO polyclonal antibody (Product # PA5-100019) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

KMO Antibody in Western Blot (WB) — Western blot analysis of KMO in mouse brain (Lane 1: treated with blocking peptide). Samples were incubated with KMO polyclonal antibody (Product # PA5-100019).

Product Details

PA5-100019

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human KMO(Accession O15229), corresponding to amino acid residues F345-A369. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2815549

Target Information

Kynurenine 3-monooxygenase (KMO; EC 1.14.13.9) is an NADPH-dependent flavin monooxygenase that catalyzes the hydroxylation of the L-tryptophan metabolite L-kynurenine to form L-3-hydroxykynurenine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: KMO; kmo {ECO:0000312|RGD:620610}; Kynurenine 3-hydroxylase; kynurenine 3-hydroxylase {ECO:0000255|HAMAP-Rule:MF_03018}; Kynurenine 3-monooxygenase; kynurenine 3-monooxygenase {ECO:0000255|HAMAP-Rule:MF_03018}

Gene Aliases: AI046660; dJ317G22.1; KMO

UniProt ID: (Human) O15229, (Mouse) Q91WN4, (Rat) O88867

Entrez Gene ID: (Human) 8564, (Mouse) 98256, (Rat) 59113

Molecular Function: oxygenase