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|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to 17 C-terminal residues of mouse Klotho.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris HCl, pH 7.5, with 0.2% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29899 detects Klotho from mouse samples.
PA1-29899 has been successfully used in Western blot and ELISA applications. PA1-29899 is expected to cross-react with human (82% identity with immunogen) due to sequence homology.
The PA1-29899 immunogen is Synthetic peptide of 17 amino acids (Mouse) (C terminal).
Animal models of human aging which display the characteristic diseases associated with aging provide insight into the cause of such diseases. Recently a trangenic mouse model, called Klotho, with several premature aging phenotypes has been described. They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of gentital organs and thymus, emphysema and short life span. KL protein associated with the Klotho mutation is a 1014 amino acid long peptide. It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane. KL gene expression was observed to be tissue specific. Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggested that KL associated aging is regulated through humoral signaling pathway. KL has been reported to be locallized on the cell surface when expressed on Cho cells. Human kl cDNA is expected to encode a protein of 1012 amino acids.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
alpha-kl; klotho; secreted form of Klotho protein