|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Pore complex-lamina fraction isolated from rat liver nuclear envelopes.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:50|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-074 detects lamin associated polypeptide (LAP) 1A, 1B and 1C from rat, human, and mouse samples. This antibody does not cross-react with LAP 2.
MA1-074 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects 3 distinct proteins of molecular weights ~75, 68 and 55 kDa representing LAP 1A, 1B and 1C, respectively in rat liver nuclear envelope fractions. Immunofluorescence staining of LAP 1 in rat liver with MA1-074 results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining.
The MA1-074 immunogen is pore complex-lamina fraction isolated from rat liver nuclear envelopes.
In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins.
The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B and 1C share a high degree of sequence homology while LAP 2 is distinct from the others. LAP 1A and B bind lamins A, C and B1, while LAP 2 binds lamin B1 only. In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton.
MA1-074 was used in western blot to study the role of SUN1 in providing a physical link between the nuclear lamina and the cytoskeleton
|Haque F,Lloyd DJ,Smallwood DT,Dent CL,Shanahan CM,Fry AM,Trembath RC,Shackleton S||Molecular and cellular biology (26:3738)||2006|
Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.
MA1-074 was used in immunocytochemistry to investigate the functional properties of lamin-binding fragment of LAP2 during the cell cycle and progression into S phase
|Yang L,Guan T,Gerace L||The Journal of cell biology (139:1077)||1997|