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Invitrogen
LAP2 Polyclonal Antibody
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FIGURE: 1 / 4
LAP2 Antibody (PA5-112215) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of LAP2 in A431 cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with LAP2 Polyclonal Antibody (Product # PA5-112215) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to Nucleus and nuclear membrane.
Product Details
PA5-112215
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived Human TMPO fragment
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B and 1C share a high degree of sequence homology while LAP 2 is distinct from the others. LAP 1A/B bind lamins A, C and B1, while LAP 2 binds lamin B1 only. In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: lamina-associated polypeptide 2; Lamina-associated polypeptide 2, isoform alpha; Lamina-associated polypeptide 2, isoforms beta/gamma; LEM domain containing 4; similar to human thymopoietin gamma encoded by GenBank Accession Number U09088; Thymopoietin isoform alpha; Thymopoietin, isoforms beta/gamma; thymopoietin-related peptide; Thymopoietin-related peptide isoform alpha; Thymopoietin-related peptide isoforms beta/gamma; TP alpha; TP beta/gamma; TPRP isoform alpha; TPRP isoforms beta/gamma; unnamed protein product
Gene Aliases: CMD1T; LAP2; LEMD4; PRO0868; TMPO; TP
UniProt ID: (Human) P42166
Entrez Gene ID: (Human) 7112
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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