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Agrisera
LFY/Leafy Polyclonal Antibody
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FIGURE: 1 / 1
LFY/Leafy Antibody (AS16-3990) in WB
20 µg of total protein: Markers (1), 35 S: : LFY lines tsp 1 (2), 35 S: : LFY lines E 1 (3), 35 S: : LFY lines tsp 2 (4), 35 S: : LFY lines E 2 (5), Empty well (6), 35 S: : LFY lines tsp 3 (7), 35 S: : LFY lines E 3 (8), LFY recombinant protein (9) extracted freshly from leaves with IP buffer (Co-IP kit from Invitrogen) and denatured with SDS reducing dye at 90°C for 5 min were separated on 12% SDS-PAGE and blotted for 10 min to PVDF using dry transfer system (i Blot, Invitrogen). Blot was blocked with blocking buffer for 10 min at RT with... View More
Product Details
AS16-3990
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from LFY sequence of Arabidopsis thaliana.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
Leafy encodes a nuclear, land plant-specific transcription factor that functions as a master regulator of floral meristem identity, promoting the transition from inflorescence to flower formation and coordinating multiple aspects of floral morphogenesis, including organ number, identity, and determinacy, in large part by activating key floral commitment and patterning programs such as APETALA1 and, in specific contexts, contributing to regulation of class B genes via cofactor interactions (e.g., with UFO at relevant promoters). The gene produces a protein with an N-terminal conserved self-association module that mediates homo-oligomerization (including a Sterile Alpha Motif-like domain implicated in multimerization) and a highly conserved C-terminal DNA-binding domain with a helix-turn-helix-like fold that binds semi-palindromic target elements as a dimer; together these domains shape genome-wide binding and transcriptional output. Beyond sequence-specific transcriptional control, LEAFY can exhibit pioneer transcription factor behavior by engaging nucleosome-occupied sites and facilitating local chromatin unlocking, supporting cell fate reprogramming toward floral identity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT5G61850; LEAFY; LEAFY 3; LFY; LFY3; MAC9.13; MAC9_13
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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