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Agrisera
LHCSR1 Polyclonal Antibody
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FIGURE: 1 / 1
LHCSR1 Antibody (AS14-2819) in WB
10 µg of a total cell extract of Chlamydomonas reinhardtii: WT _P _HL_High CO 2 (1), WT_P_HL (2), Npq 4_P _HL (3), were separated on Bolt® 4-12% Bis-Tris Plus Gels (precast) and blotted to PVDF using Bolt® transfer system for 1 h. Blots were blocked with 5% BSA/milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-... View More
Product Details
AS14-2819
Product Specifications
Species Reactivity
Algae
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from LHCSR1 protein sequence from Chlamydomonas reinhardtii.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Bryopsis corticulans, Chlamydomonas reinhardtii, Nannochloropsis gaditana
Target Information
LHCSR1 encodes a chloroplast thylakoid membrane light-harvesting complex stress-related protein that accumulates in photosynthetic membranes where it associates with antenna complexes of the photosystems. LHCSR1 is a small integral membrane pigment-binding protein of the LHC superfamily predicted to form a multi-helix (typically three transmembrane helices) fold with lumen-exposed protonatable residues and conserved chlorophyll/carotenoid-binding features that enable rapid conformational switching. Biologically, LHCSR1 is a key effector of non-photochemical quenching (NPQ), acting as a luminal pH sensor and quencher that converts excess absorbed excitation energy into heat to limit photo-oxidative damage; in reconstituted and cellular systems, small amounts of LHCSR1 can induce large, fast, and reversible low-pH-dependent quenching of LHCII, and its quenching behavior is further modulated by carotenoid composition (including zeaxanthin), supporting dual control by lumen acidification and xanthophyll binding to tune photoprotection under excess or fluctuating light.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CHLRE_02g080650v5; CHLREDRAFT_97057; Cre02.g080650; lhcSR1
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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