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LIG4 Polyclonal Antibody

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LIG4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 12695-1-AP (LIG4 antibody) at dilution of 1:100 (under 10x lens).

LIG4 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

12695-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LIG4 Fusion Protein Ag3385 (606-911 aa encoded by BC037491) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: ASGKLASKH LYIGGDDEPQ EKKRKAAPKM KKVIGIIEHL KAPNLTNVNK ISNIFEDVEF CVMSGTDSQP KPDLENRIAE FGGYIVQNPG PDTYCVIAGS ENIRVKNIIL SNKHDVVKPA WLLECFKTKS FVPWQPRFMI HMCPSTKEHF AREYDCYGDS YFIDTDLNQL KEVFSGIKNS NEQTPEEMAS LIADLEYRYS WDCSPLSMFR RHTVYLDSYA VINDLSTKNE GTRLAIKALE LRFHGAKVVS CLAEGVSHVI IGEDHSRVAD FKAFRRTFKR KFKILKESWV TDSIDKCELQ EENQYLI (606-911 aa encoded by BC037491 )

Target Information

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Berberine activates caspase-9/cytochrome c-mediated apoptosis to suppress triple-negative breast cancer cells in vitro and in vivo.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhao Y,Jing Z,Lv J,Zhang Z,Lin J,Cao X,Zhao Z,Liu P,Mao W

Wed Nov 01 00:00:00 EDT 2017

XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency.

The Journal of allergy and clinical immunology

Guo C,Nakazawa Y,Woodbine L,Björkman A,Shimada M,Fawcett H,Jia N,Ohyama K,Li TS,Nagayama Y,Mitsutake N,Pan-Hammarström Q,Gennery AR,Lehmann AR,Jeggo PA,Ogi T

Thu Oct 01 00:00:00 EDT 2015

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Toxicology and applied pharmacology

Dong H,Shi Q,Song X,Fu J,Hu L,Xu D,Su C,Xia X,Song E,Song Y

Wed Jul 01 00:00:00 EDT 2015

Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway.

Nature communications

Xing M,Yang M,Huo W,Feng F,Wei L,Jiang W,Ning S,Yan Z,Li W,Wang Q,Hou M,Dong C,Guo R,Gao G,Ji J,Zha S,Lan L,Liang H,Xu D

Wed Feb 11 00:00:00 EST 2015

Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage.

PloS one

Cousin W,Ho ML,Desai R,Tham A,Chen RY,Kung S,Elabd C,Conboy IM

Mon Dec 30 00:00:00 EST 2013

Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication.

Cell host & microbe

Paran N,De Silva FS,Senkevich TG,Moss B

Thu Dec 17 00:00:00 EST 2009

Bioinformatics

Protein Aliases: DNA joinase; DNA ligase (ATP) 4; DNA ligase 4; DNA ligase IV; DNA repair enzyme; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase

Gene Aliases: 5830471N16Rik; LIG4; LIG4S; tiny

UniProt ID: (Human) P49917, (Mouse) Q8BTF7

Entrez Gene ID: (Human) 3981, (Rat) 290907, (Mouse) 319583

Molecular Function: DNA metabolism protein