Proteintech

LIG4 Polyclonal Antibody

LIG4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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LIG4 Antibody (12695-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human prostate cancer using 12695-1-AP (LIG4 antibody) at dilution of 1:100 (under 10x lens).

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Product Details

12695-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 20 publications 20 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LIG4 Fusion Protein Ag3385 (606-911 aa encoded by BC037491)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ASGKLASKH LYIGGDDEPQ EKKRKAAPKM KKVIGIIEHL KAPNLTNVNK ISNIFEDVEF CVMSGTDSQP KPDLENRIAE FGGYIVQNPG PDTYCVIAGS ENIRVKNIIL SNKHDVVKPA WLLECFKTKS FVPWQPRFMI HMCPSTKEHF AREYDCYGDS YFIDTDLNQL KEVFSGIKNS NEQTPEEMAS LIADLEYRYS WDCSPLSMFR RHTVYLDSYA VINDLSTKNE GTRLAIKALE LRFHGAKVVS CLAEGVSHVI IGEDHSRVAD FKAFRRTFKR KFKILKESWV TDSIDKCELQ EENQYLI (606-911 aa encoded by BC037491 )

Target Information

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

C9orf72 functions in the nucleus to regulate DNA damage repair.

Cell death and differentiation

He L,Liang J,Chen C,Chen J,Shen Y,Sun S,Li L

Wed Mar 01 00:00:00 UTC 2023

Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation.

International journal of biological macromolecules

Tao HY,He SM,Zhao CY,Wang Y,Sheng WJ,Zhen YS

Tue Jan 31 00:00:00 UTC 2023

Comparison of DNA stability and its related genes of neurons derived from induced pluripotent stem cells and primary retinal neurons.

Cell biology international

Yu N,Qiu J,Li K,Luo Q,Liu X,Yang Y,Jiang Z,He S,He A,Chen S,Chen X,Li Y,Ge J,Zhuang J,Yu K

Sat Oct 01 00:00:00 UTC 2022

Comparison of DNA stability and its related genes of neurons derived from induced pluripotent stem cells and primary retinal neurons.

Cell biology international

Yu N,Qiu J,Li K,Luo Q,Liu X,Yang Y,Jiang Z,He S,He A,Chen S,Chen X,Li Y,Ge J,Zhuang J,Yu K

Sat Oct 01 00:00:00 UTC 2022

Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.

Nature communications

Guo G,Gao M,Gao X,Zhu B,Huang J,Tu X,Kim W,Zhao F,Zhou Q,Zhu S,Wu Z,Yan Y,Zhang Y,Zeng X,Zhu Q,Yin P,Luo K,Sun J,Deng M,Lou Z

Mon Apr 12 00:00:00 UTC 2021

Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining.

Cancer research

Zhou Q,Howard ME,Tu X,Zhu Q,Denbeigh JM,Remmes NB,Herman MG,Beltran CJ,Yuan J,Greipp PT,Boughey JC,Wang L,Johnson N,Goetz MP,Sarkaria JN,Lou Z,Mutter RW

Tue Jun 15 00:00:00 UTC 2021

CRISPR/Cas9 library screening uncovered methylated PKP2 as a critical driver of lung cancer radioresistance by stabilizing β-catenin.

Oncogene

Cheng C,Pei X,Li SW,Yang J,Li C,Tang J,Hu K,Huang G,Min WP,Sang Y

Thu Apr 01 00:00:00 UTC 2021

MiR-1587 Regulates DNA Damage Repair and the Radiosensitivity of CRC Cells via Targeting LIG4.

Dose-response : a publication of International Hormesis Society

Liu R,Shen L,Lin C,He J,Wang Q,Qi Z,Zhang Q,Zhou M,Wang Z

Mon Sep 28 00:00:00 UTC 2020

Long noncoding RNA lnc-RI regulates DNA damage repair and radiation sensitivity of CRC cells through NHEJ pathway.

Cell biology and toxicology

Liu R,Zhang Q,Shen L,Chen S,He J,Wang D,Wang Q,Qi Z,Zhou M,Wang Z

Thu Oct 01 00:00:00 UTC 2020

RNase L facilitates the repair of DNA double-strand breaks through the nonhomologous end-joining pathway.

FEBS letters

Zhong Y,Pan B,Zhu J,Fu H,Zheng X

Sat Jun 01 00:00:00 UTC 2019

XRCC4, which is inhibited by PFDA, regulates DNA damage repair and cell chemosensitivity.

Journal of cellular biochemistry

Liu F,Fan Z,Song N,Han M,Yan M,Guo LH,Jihui J,Liu S

Thu Aug 01 00:00:00 UTC 2019

Retinal Neuron Is More Sensitive to Blue Light-Induced Damage than Glia Cell Due to DNA Double-Strand Breaks.

Cells

Chen P,Lai Z,Wu Y,Xu L,Cai X,Qiu J,Yang P,Yang M,Zhou P,Zhuang J,Ge J,Yu K,Zhuang J

Fri Jan 18 00:00:00 UTC 2019

MiR-34s negatively regulate homologous recombination through targeting RAD51.

Archives of biochemistry and biophysics

Chen S,Liu R,Wang Q,Qi Z,Hu Y,Zhou P,Wang Z

Wed May 15 00:00:00 UTC 2019

Rap1 regulates hematopoietic stem cell survival and affects oncogenesis and response to chemotherapy.

Nature communications

Khattar E,Maung KZY,Chew CL,Ghosh A,Mok MMH,Lee P,Zhang J,Chor WHJ,Cildir G,Wang CQ,Mohd-Ismail NK,Chin DWL,Lee SC,Yang H,Shin YJ,Nam DH,Chen L,Kumar AP,Deng LW,Ikawa M,Gunaratne J,Osato M,Tergaonkar V

Fri Dec 13 00:00:00 UTC 2019

Exosome-packaged miR-1246 contributes to bystander DNA damage by targeting LIG4.

British journal of cancer

Mo LJ,Song M,Huang QH,Guan H,Liu XD,Xie DF,Huang B,Huang RX,Zhou PK

Wed Aug 01 00:00:00 UTC 2018

Berberine activates caspase-9/cytochrome c-mediated apoptosis to suppress triple-negative breast cancer cells in vitro and in vivo.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhao Y,Jing Z,Lv J,Zhang Z,Lin J,Cao X,Zhao Z,Liu P,Mao W

Wed Nov 01 00:00:00 UTC 2017

Berberine activates caspase-9/cytochrome c-mediated apoptosis to suppress triple-negative breast cancer cells in vitro and in vivo.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhao Y,Jing Z,Lv J,Zhang Z,Lin J,Cao X,Zhao Z,Liu P,Mao W

Wed Nov 01 00:00:00 UTC 2017

XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency.

The Journal of allergy and clinical immunology

Guo C,Nakazawa Y,Woodbine L,Björkman A,Shimada M,Fawcett H,Jia N,Ohyama K,Li TS,Nagayama Y,Mitsutake N,Pan-Hammarström Q,Gennery AR,Lehmann AR,Jeggo PA,Ogi T

Thu Oct 01 00:00:00 UTC 2015

XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency.

The Journal of allergy and clinical immunology

Guo C,Nakazawa Y,Woodbine L,Björkman A,Shimada M,Fawcett H,Jia N,Ohyama K,Li TS,Nagayama Y,Mitsutake N,Pan-Hammarström Q,Gennery AR,Lehmann AR,Jeggo PA,Ogi T

Thu Oct 01 00:00:00 UTC 2015

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Toxicology and applied pharmacology

Dong H,Shi Q,Song X,Fu J,Hu L,Xu D,Su C,Xia X,Song E,Song Y

Wed Jul 01 00:00:00 UTC 2015

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Toxicology and applied pharmacology

Dong H,Shi Q,Song X,Fu J,Hu L,Xu D,Su C,Xia X,Song E,Song Y

Wed Jul 01 00:00:00 UTC 2015

Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway.

Nature communications

Xing M,Yang M,Huo W,Feng F,Wei L,Jiang W,Ning S,Yan Z,Li W,Wang Q,Hou M,Dong C,Guo R,Gao G,Ji J,Zha S,Lan L,Liang H,Xu D

Wed Feb 11 00:00:00 UTC 2015

Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway.

Nature communications

Xing M,Yang M,Huo W,Feng F,Wei L,Jiang W,Ning S,Yan Z,Li W,Wang Q,Hou M,Dong C,Guo R,Gao G,Ji J,Zha S,Lan L,Liang H,Xu D

Wed Feb 11 00:00:00 UTC 2015

Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage.

PloS one

Cousin W,Ho ML,Desai R,Tham A,Chen RY,Kung S,Elabd C,Conboy IM

Mon Dec 30 00:00:00 UTC 2013

Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage.

PloS one

Cousin W,Ho ML,Desai R,Tham A,Chen RY,Kung S,Elabd C,Conboy IM

Mon Dec 30 00:00:00 UTC 2013

Differences in sensitivity to DNA-damaging Agents between XRCC4- and Artemis-deficient human cells.

Journal of radiation research

Katsube T,Mori M,Tsuji H,Shiomi T,Shiomi N,Onoda M

Mon Jan 09 00:00:00 UTC 2012

Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication.

Cell host & microbe

Paran N,De Silva FS,Senkevich TG,Moss B

Thu Dec 17 00:00:00 UTC 2009

Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication.

Cell host & microbe

Paran N,De Silva FS,Senkevich TG,Moss B

Thu Dec 17 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: DNA joinase; DNA ligase (ATP) 4; DNA ligase 4; DNA ligase IV; DNA repair enzyme; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase

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Gene Aliases: 5830471N16Rik; LIG4; LIG4S; tiny

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UniProt ID: (Human) P49917, (Mouse) Q8BTF7

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Entrez Gene ID: (Human) 3981, (Rat) 290907, (Mouse) 319583

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Function(s)

DNA binding DNA ligase activity DNA ligase (ATP) activity protein binding ATP binding protein C-terminus binding ligase activity metal ion binding nucleotide binding DNA metabolism protein

Process(es)

single strand break repair in utero embryonic development pro-B cell differentiation DNA ligation lagging strand elongation nucleotide-excision repair, DNA gap filling double-strand break repair double-strand break repair via nonhomologous end joining cell cycle central nervous system development cell proliferation response to X-ray response to gamma radiation T cell differentiation in thymus V(D)J recombination immunoglobulin V(D)J recombination T cell receptor V(D)J recombination somatic stem cell population maintenance negative regulation of neuron apoptotic process isotype switching positive regulation of fibroblast proliferation positive regulation of neurogenesis DNA ligation involved in DNA recombination DNA ligation involved in DNA repair chromosome organization cell division neuron apoptotic process cellular response to lithium ion DNA biosynthetic process establishment of integrated proviral latency double-strand break repair via classical nonhomologous end joining positive regulation of chromosome organization DNA repair nucleotide-excision repair response to ionizing radiation DNA replication DNA recombination cellular response to DNA damage stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LIG4 Polyclonal Antibody

LIG4 Antibody (12695-1-AP) in IHC (P)

Product Details

12695-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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