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Invitrogen
LIN28A Polyclonal Antibody
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FIGURE: 1 / 1
LIN28A Antibody (PA5-72262) in WB
Western blot analysis of LIN28A in various lysates. Samples were incubated with LIN28A polyclonal antibody (Product # PA5-72262) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Zebrafish muscle lysates; Lane 2: Zebrafish lysates. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-72262
Product Specifications
Species Reactivity
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 85-119 amino acids from the Central region of DANRE lin28a
Conjugate
Form
Liquid
Concentration
0.45 mg/mL
Amount
90 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ12457; Lin-28A; Protein lin-28 homolog A; RNA-binding protein lin-28
Gene Aliases: lin28; lin28a; sb:cb650; si:ch211-232d9.4; zgc:55584
UniProt ID: (Zebrafish) Q803L0
Entrez Gene ID: (Zebrafish) 394066
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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