Proteintech

LMNB2 Polyclonal Antibody

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LMNB2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 10895-1-AP (LMNB2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

LMNB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 10895-1-AP (LMNB2 antibody) at dilution of 1:100 (under 10x lens).

LMNB2 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10895-1-AP (LMNB2 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

LMNB2 Antibody in Flow Cytometry (Flow) — 1X10^6 HEK-293T cells were stained with 0.2ug LMNB2 antibody (10895-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

LMNB2 Antibody in Immunoprecipitation (IP) — IP result of anti-LMNB2 (IP:10895-1-AP, 3ug; Detection:10895-1-AP 1:500) with Jurkat cells lysate 2400ug.

Product Details

10895-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 2 publications 2 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LMNB2 Fusion Protein Ag1335 (241-600 aa encoded by BC006551) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: EELRSQHDE QVRLYKLELE QTYQAKLDSA KLSSDQNDKA ASAAREELKE ARMRLESLSY QLSGLQKQAS AAEDRIRELE EAMAGERDKF RKMLDAKEQE MTEMRDVMQQ QLAEYQELLD VKLALDMEIN AYRKLLEGEE ERLKLSPSPS SRVTVSRATS SSSGSLSATG RLGRSKRKRL EVEEPLGSGP SVLGTGTGGS GGFHLAQQAS ASGSVSIEEI DLEGKFVQLK NNSDKDQSLG NWRIKRQVLE GEEIAYKFTP KYILRAGQMV TVWAAGAGVA HSPPSTLVWK GQSSWGTGES FRTVLVNADG EEVAMRTVKK SSVMRENENG EEEEEEAEFG EEDLFHQQGD PRTTSRGCYV M (241-600 aa encoded by BC006551)

Target Information

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.