Invitrogen

LMX1A Polyclonal Antibody

View all (4) LMX1A antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

LMX1A Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of LMX1A in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115517) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

LMX1A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of LMX1A in human pancreatic cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115517) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

LMX1A Antibody in Western Blot (WB) — Western blot analysis of LMX1A in rat brain (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115517).

Product Details

PA5-115517

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human LMX1A(Accession Q8TE12), corresponding to amino acid residues S165-A186.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2900153

Target Information

The LIM homeobox transcription factor 1 (LMX1A) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1A binds an A/T-rich sequence and functions in the nucleus as a transcriptional activator to the insulin gene promoter. In the developing embryo, LMX1A is expressed along the neuraxis and leads to the development of the roof plate of the vertebrae. While LMX1A is crucial for the differentiation of dopamine neurons, the related LMX1B appears to be important for both the development and the survival of these cells. It has been suggested that LMX1A might be a useful biomarker or risk factor in tumor aggressiveness.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: LIM homeobox transcription factor 1, alpha; LIM homeobox transcription factor 1-alpha; LIM/homeobox protein 1.1; LIM/homeobox protein LMX1A; LMX-1; LMX-1.1; MGC87616; shaker short-tail

Gene Aliases: dr; dreher; LMX1; LMX1.1; LMX1A; sst

UniProt ID: (Human) Q8TE12, (Mouse) Q9JKU8

Entrez Gene ID: (Human) 4009, (Rat) 289201, (Mouse) 110648

Function(s)