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LNX2 Antibody (720004) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized HCT 116 cells for detection of LNX2 using Anti-LNX2 Rabbit Polyclonal Antibody (Product # 720004, 3 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of LNX2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50). Panel... View More
Product Details
720004
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Multiple peptides against Human LNX2 (aa 442-458, 129-145, 671-683)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 30% glycerol, 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2532784
Product Specific Information
This antibody is predicted to react with Goat, Bovine, Horse, Pig, Cat and Rabbit.
Target Information
LNX2 (Ligand of Numb Protein X 2) protein is encoded by the LNX2 gene located on chromosome 13 in humans and is known as PDZRN1 (PDZ domain-containing RING finger 1). It is through its PDZ domains that LNX2 forms oligomers and interacts with some of its target proteins. LNX2 is best studied as a E3 ubiquitin ligase that also contains the NPXY motifs which allows binding to the Numb protein. This interaction has been shown to be responsible for ubiquitination of Numb, which prevents the proteasomal degradation of Notch intracellular domain (NICD) and thus modulates the Notch signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ligand of Numb protein X 2; Numb-binding protein 2; PDZ domain containing ring finger 1; PDZ domain-containing RING finger protein 1
Gene Aliases: 9630046H24; AW209022; LNX2; PDZRN1
UniProt ID: (Human) Q8N448, (Mouse) Q91XL2
Entrez Gene ID: (Human) 222484, (Mouse) 140887, (Rat) 360761
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