Immunofluorescent analysis of LRP130 showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a LRP130 polyclonal antibody (Product # PA5-22034) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 611 and 937 of LRP130 (Uniprot ID#P42704)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
PA5-22034 targets LRP130 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-22034 immunogen is a recombinant fragment corresponding to a region within amino acids 611 and 937 of LRP130 (Uniprot ID#P42704).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The BRCA1 tumor suppressor binds to inositol 1,4,5-trisphosphate receptors to stimulate apoptotic calcium release.
PA5-22034 was used in immunoprecipitation to study the interaction between BRCA1 tumor suppressor and inositol 1,4,5-trisphosphate receptors and its effect on apoptotic calcium release
|Hedgepeth SC,Garcia MI,Wagner LE,Rodriguez AM,Chintapalli SV,Snyder RR,Hankins GD,Henderson BR,Brodie KM,Yule DI,van Rossum DB,Boehning D||The Journal of biological chemistry (290:7304)||2015|