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Western blot analysis of LSM4 in (lane 2) Gene specific transient over-expression HEK293T cell lysate and (lane 3) Empty vector transfected control HEK293T cell lysate. Samples were probed with a LSM4 polyclonal antibody (Product # PA1-28314) at a dilution of 1:1000. Secondary detection was performed using a peroxidase conjugated Rabbit anti-Chicken IgY (IgG) at a 1:10,000 dilution and developed with TMB Substrate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Recombinant fragment, corresponding to amino acids 1-139 of Human LSM4.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28314 detects LSM4 from mouse, human, rat samples.
PA1-28314 has been successfully used in Western blot applications.
The PA1-28314 immunogen is: Recombinant fragment, corresponding to amino acids 1-139 of Human LSM4.
Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
glycine-rich protein; LSM4 homolog, U6 small nuclear RNA associated; LSM4 U6 small nuclear RNA and mRNA degradation associated; U6 snRNA-associated SM-like protein 4; U6 snRNA-associated Sm-like protein LSm4; YER112W
GRP; LSM4; YER112W