Proteintech

LSS Polyclonal Antibody

LSS Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

LSS Antibody (13715-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 13715-1-AP (LSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

LSS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LSS Antibody in Immunoprecipitation (IP)

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Product Details

13715-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LSS Fusion Protein Ag4655 (382-732 aa encoded by BC035638)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can recognize all these three isoforms.

Immunogen sequence: NGSQIWDTA FAIQALLEAG GHHRPEFSSC LQKAHEFLRL SQVPDNPPDY QKYYRQMRKG GFSFSTLDCG WIVSDCTAEA LKAVLLLQEK CPHVTEHIPR ERLCDAVAVL LNMRNPDGGF ATYETKRGGH LLELLNPSEV FGDIMIDYTY VECTSAVMQA LKYFHKRFPE HRAAEIRETL TQGLEFCRRQ QRADGSWEGS WGVCFTYGTW FGLEAFACMG QTYRDGTACA EVSRACDFLL SRQMADGGWG EDFESCEERR YVQSAQSQIH NTCWAMMGLM AVRHPDIEAQ ERGVRCLLEK QLPNGDWPQE NIAGVFNKSC AISYTSYRNI FPIWALGRFS QLYPERALAG HP (382-732 aa encoded by BC035638)

Target Information

The protein encoded by this gene catalyzes the conversion of (S)-2, 3 oxidosqualene to lanosterol. The encoded protein is a member of the terpene cyclase/mutase family and catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sinapic and ferulic acid phenethyl esters increase the expression of steroidogenic genes in MA-10 tumor Leydig cells.

Toxicology in vitro : an international journal published in association with BIBRA

Basque A,Touaibia M,Martin LJ

Wed Feb 01 00:00:00 UTC 2023

Hypoxia-altered cholesterol homeostasis enhances the expression of interferon-stimulated genes upon SARS-CoV-2 infections in monocytes.

Frontiers in immunology

Bauer R,Meyer SP,Raue R,Palmer MA,Guerrero Ruiz VM,Cardamone G,Rösser S,Heffels M,Roesmann F,Wilhelm A,Lütjohann D,Zarnack K,Fuhrmann DC,Widera M,Schmid T,Brüne B

Fri Jun 30 00:00:00 UTC 2023

Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Xu XY,Choi HS,Park SY,Kim JK,Seo KH,Kim H,Kim YJ

Sat Jan 01 00:00:00 UTC 2022

Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Xu XY,Choi HS,Park SY,Kim JK,Seo KH,Kim H,Kim YJ

Sat Jan 01 00:00:00 UTC 2022

Design, synthesis and neurite outgrowth activity of novel ganglioside GM1 derivatives by remodeling of the fatty acid moiety.

European journal of medicinal chemistry

Chen X,Jin X,Huang F,Wang J,Cao X,Wang PG,Feng Y,Jiang F,Yang G

Sat Nov 05 00:00:00 UTC 2022

Biallelic Variants in Lanosterol Synthase (LSS) Cause Palmoplantar Keratoderma-Congenital Alopecia Syndrome Type 2.

The Journal of investigative dermatology

Yang F,Jiang X,Zhu Y,Lee M,Xu Z,Zhang J,Li Q,Lin MY,Wang H,Lin Z

Sat Oct 01 00:00:00 UTC 2022

Gigantol Improves Cholesterol Metabolism and Progesterone Biosynthesis in MA-10 Leydig Cells.

Current issues in molecular biology

Basque A,Nguyen HT,Touaibia M,Martin LJ

Thu Dec 23 00:00:00 UTC 2021

The antiviral enzyme viperin inhibits cholesterol biosynthesis.

The Journal of biological chemistry

Grunkemeyer TJ,Ghosh S,Patel AM,Sajja K,Windak J,Basrur V,Kim Y,Nesvizhskii AI,Kennedy RT,Marsh ENG

Thu Jul 01 00:00:00 UTC 2021

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Neuron

Li X,Zhang J,Li D,He C,He K,Xue T,Wan L,Zhang C,Liu Q

Wed Mar 17 00:00:00 UTC 2021

Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice.

PLoS genetics

Wada Y,Kikuchi A,Kaga A,Shimizu N,Ito J,Onuma R,Fujishima F,Totsune E,Sato R,Niihori T,Shirota M,Funayama R,Sato K,Nakazawa T,Nakayama K,Aoki Y,Aiba S,Nakagawa K,Kure S

Sat Feb 01 00:00:00 UTC 2020

Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice.

PLoS genetics

Wada Y,Kikuchi A,Kaga A,Shimizu N,Ito J,Onuma R,Fujishima F,Totsune E,Sato R,Niihori T,Shirota M,Funayama R,Sato K,Nakazawa T,Nakayama K,Aoki Y,Aiba S,Nakagawa K,Kure S

Sat Feb 01 00:00:00 UTC 2020

Pharmacogenomics of Chemically Distinct Classes of Keap1-Nrf2 Activators Identify Common and Unique Gene, Protein, and Pathway Responses In Vivo.

Molecular pharmacology

Wible RS,Tran QT,Fathima S,Sutter CH,Kensler TW,Sutter TR

Sun Apr 01 00:00:00 UTC 2018

Pharmacogenomics of Chemically Distinct Classes of Keap1-Nrf2 Activators Identify Common and Unique Gene, Protein, and Pathway Responses In Vivo.

Molecular pharmacology

Wible RS,Tran QT,Fathima S,Sutter CH,Kensler TW,Sutter TR

Sun Apr 01 00:00:00 UTC 2018

The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis.

The Journal of biological chemistry

Fryer LG,Jones B,Duncan EJ,Hutchison CE,Ozkan T,Williams PA,Alder O,Nieuwdorp M,Townley AK,Mensenkamp AR,Stephens DJ,Dallinga-Thie GM,Shoulders CC

Fri Feb 14 00:00:00 UTC 2014

The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis.

The Journal of biological chemistry

Fryer LG,Jones B,Duncan EJ,Hutchison CE,Ozkan T,Williams PA,Alder O,Nieuwdorp M,Townley AK,Mensenkamp AR,Stephens DJ,Dallinga-Thie GM,Shoulders CC

Fri Feb 14 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: 2,3-epoxysqualene--lanosterol cyclase; 2,3-epoxysqualene-lanosterol cyclase; 2,3-oxidosqualene: lanosterol cyclase; hOSC; Lanosterol synthase; OSC; Oxidosqualene--lanosterol cyclase

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Gene Aliases: 2810025N20Rik; CTRCT44; D10Ertd116e; LSS; OSC

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UniProt ID: (Human) P48449, (Mouse) Q8BLN5, (Rat) P48450

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Entrez Gene ID: (Human) 4047, (Mouse) 16987, (Rat) 81681

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Function(s)

lanosterol synthase activity intramolecular transferase activity cyclase

Process(es)

steroid biosynthetic process cholesterol biosynthetic process regulation of protein stability metabolic process sterol metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LSS Polyclonal Antibody

LSS Antibody (13715-1-AP) in ICC/IF

Product Details

13715-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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