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Agrisera
LUC/Luciferase Polyclonal Antibody
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FIGURE: 1 / 1
LUC/Luciferase Antibody (AS16-3691) in WB
Total and cytosolic extract from Arabidopsis thaliana seedlings with detectable Luciferase expression at different zt time, three samples of a line with silenced luciferase expression (no detection of Luciferin caused light emission) and one wild-type without CAB 2:: LUC expression. 100 µg of tissue was homogenized with extraction buffer on ice, spun at 4°C at 20,000 g/15 min. and denatured at 100°C or 10 min. Samples were separated on 12% SDS-PAGE and blotted over night to PVDF using semi-dry. Blots were blocked with milk-TBS for 1 h at r... View More
Product Details
AS16-3691
Product Specifications
Species Reactivity
Luciferase
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from N-terminal part of LUC protein sequence of Photinus pyralis.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Specific Species Reactivity: LUC
Target Information
Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Firefly Luciferase; Fluc; Genji firefly; Green Luciferase; Japanese firefly; luc; LUC1; luciferin 4-monooxygenase; Photinus pyralis; photinus pyralis luciferase; Pp-Luc; PpyLuc1
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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