Active Motif

LXR-beta Polyclonal Antibody

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LXR-beta Antibody in Western Blot (WB) — LXR-beta pAb tested by Western blot. Detection of LXR-beta by Western blot analysis. HeLa whole-cell extract (20 µg) was probed with LXR-beta pAb at a 1:1000 dilution.

LXR-beta Antibody in ChIP Assay (ChIP) — LXR-beta pAb tested by ChIP-qPCR. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 25 µg of human liver chromatin and 10 µl of LXR-beta antibody. ChIP DNA was used in qPCR with the negative control primer pairs and primers against the known LXR binding site in the RARB promoter. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

Product Details

61177

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

ChIP assay (ChIP)

10 µL

View 1 publication 1 publication

ChIP-sequencing (ChIP-Seq)

10 µL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This LXR-beta antibody was raised against a peptide in the N-terminal region of human LXR-beta.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.5, with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2614980

Target Information

The liver X receptors, LXRA and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors and regulate expression of target genes containing LXR response elements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Distinct gene regulatory programs define the inhibitory effects of liver X receptors and PPARG on cancer cell proliferation.

Genome medicine

Savic D,Ramaker RC,Roberts BS,Dean EC,Burwell TC,Meadows SK,Cooper SJ,Garabedian MJ,Gertz J,Myers RM

Research topic: Cancer

Mon Jul 11 00:00:00 EDT 2016

Broad-spectrum therapeutic suppression of metastatic melanoma through nuclear hormone receptor activation.

Cell

Pencheva N,Buss CG,Posada J,Merghoub T,Tavazoie SF

Research topic: Melanoma

Thu Feb 27 00:00:00 EST 2014

Bioinformatics

Protein Aliases: liver X nuclear receptor beta; Liver X receptor beta; LX receptor beta; nuclear orphan receptor LXR-beta; Nuclear receptor NER; Nuclear receptor subfamily 1 group H member 2; nuclear receptor subfamily 1, group H, member 2; Oxysterols receptor LXR-beta; steroid hormone-nuclear receptor NER; Ubiquitously-expressed nuclear receptor

Gene Aliases: LXR-b; LXRB; NER; NER-I; NR1H2; RIP15; UNR

UniProt ID: (Human) P55055

Entrez Gene ID: (Human) 7376

Function(s)

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor activity, sequence-specific transcription regulatory region DNA binding DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding zinc ion binding apolipoprotein A-I receptor binding retinoid X receptor binding ATPase binding