Invitrogen

LYPLA3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (5) LYPLA3 antibodies

Datasheet

Datasheet

Product Image Gallery

LYPLA3 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of LYPLA3 in human colon, lymph node, pancreas and placenta using LYPLA3 Polyclonal Antibody (Product # PA5-59682) (A) shows similar protein distribution across tissues to an independent LYPLA3 Polyclonal Antibody (B).

LYPLA3 Antibody in Western Blot (WB) — Western blot analysis of LYPLA3 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a LYPLA3 Polyclonal Antibody (Product # PA5-59682).

LYPLA3 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of LYPLA3 demonstrates antibody specificity. Immunohistochemical analysis of LYPLA3 using anti-LYPLA3 Polyclonal Antibody (Product # PA5-59682), shows significant staining of LYPLA3 in human placenta and shows minimal or weak staining in human pancreas tissues. The relative expression levels of LYPLA3 within each tissue is shown using RNA-Seq. {RE}

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human LYPLA3

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2643631

Product Specific Information

Immunogen sequence: GVAKTLRVLA SGDNNRIPVI GPLKIREQQR SAVSTSWLLP YNYTWSPEKV FVQTPTINYT LRDYRKFFQD IGFED

Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 92%.

Target Information

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 1-O-acylceramide synthase; 1-O-acylceramide synthase {ECO:0000303|PubMed:15294901}; ACS; ACS {ECO:0000303|PubMed:15294901}; Group XV phospholipase A2; group XV phospholipase A2 {ECO:0000312|EMBL:AAH98894.1}; GXVPLA2; LCAT-like lysophospholipase; LCAT-like lysophospholipase {ECO:0000250|UniProtKB:Q8VEB4}; LLPL; LLPL {ECO:0000250|UniProtKB:Q8VEB4}; LPLA2; LPLA2 {ECO:0000303|PubMed:15294901}; LYPLA3; Lysophospholipase 3; lysophospholipase 3 (lysosomal phospholipase A2); lysophospholipase 3 {ECO:0000250|UniProtKB:Q8VEB4}; Lysosomal phospholipase A2; lysosomal phospholipase A2 {ECO:0000303|PubMed:15294901, ECO:0000312|EMBL:AAS66767.1}; phospholipase A2, group XV; pla2g15 {ECO:0000312|Ensembl:ENSRNOP00000026996, ECO:0000312|RGD:1302982}

Gene Aliases: 1-O-acylceramidesynthase; ACS; C87498; GXVPLA2; LLPL; LPLA2; LYPLA3; PLA2G15; UNQ341/PRO540

UniProt ID: (Human) Q8NCC3, (Mouse) Q8VEB4, (Rat) Q675A5

Entrez Gene ID: (Human) 23659, (Mouse) 192654, (Rat) 361401

Molecular Function: acyltransferase hydrolase lipase phospholipase transferase