Western blot analysis of Lubricin was performed by loading 3µg of non-reduced human synovial fluid per well onto a SDS-PAGE gel. Proteins were transferred to a membrane. The membrane was probed with a Lubricin polyclonal antibody (Product # PA3-118) at a concentration of 2µg/ml diluted in the antibody diluent provided in the Fast Western Blot Kit (Product # 35050), followed by the optimized HRP reagent from the same kit (diluted by adding 150µl of HRP reagent to 10ml of reagent diluent). Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C L(1356) P N I R K Q P D G Y D Y Y A F S K D Q(1374) of human Lubricin.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-118 detects Lubricin in human samples.
PA3-118 has been successfully used in Western blot procedures. By Western blot, this antibody detects a 280 kDa protein representing recombinant human Lubricin. PA3-118 works best on purified or recombinant samples under non-reducing conditions.
PA3-118 immunogen is a synthetic peptide corresponding to residues C L(1356) P N I R K Q P D G Y D Y Y A F S K D Q(1374) of human Lubricin. This polyclonal antibody has been referred to as: JSCLPN.
Lubricin is a glycoprotein encoded by the PRG4 gene. It has been identified as a component of joint lubrication and synovial homeostasis by reducing friction in joints and preventing synovial cell overgrowth. It is a large, water soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of lubricin function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.