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Invitrogen
Lubricin Polyclonal Antibody
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FIGURE: 1 / 6
Lubricin Antibody (PA3-118) in WB
Western blot analysis of Lubricin was performed by loading 3 µg of non-reduced human synovial fluid per well onto a SDS-PAGE gel. Proteins were transferred to a membrane. The membrane was probed with a Lubricin polyclonal antibody (Product # PA3-118) at a concentration of 2 µg/mL diluted in the antibody diluent provided in the Fast Western Blot Kit (Product # 35050), followed by the optimized HRP reagent from the same kit (diluted by adding 150 µL of HRP reagent to 10 mL of reagent diluent). Chemiluminescent detection was performed using S... View More
Product Details
PA3-118
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Horse,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C L(1356) P N I R K Q P D G Y D Y Y A F S K D Q(1374) of human Lubricin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-118 detects Lubricin in human samples.
PA3-118 has been successfully used in Western blot procedures. By Western blot, this antibody detects a 280 kDa protein representing recombinant human Lubricin. PA3-118 works best on purified or recombinant samples under non-reducing conditions.
PA3-118 immunogen is a synthetic peptide corresponding to residues C L(1356) P N I R K Q P D G Y D Y Y A F S K D Q(1374) of human Lubricin. This polyclonal antibody has been referred to as: JSCLPN.
Target Information
Lubricin is a glycoprotein encoded by the PRG4 gene. It has been identified as a component of joint lubrication and synovial homeostasis by reducing friction in joints and preventing synovial cell overgrowth. It is a large, water soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of lubricin function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: articular superficial zone protein; hemangiopoietin; Lubricin; megakaryocyte stimulating factor; Megakaryocyte-stimulating factor; Proteoglycan 4; Proteoglycan-4; Superficial zone proteoglycan; unnamed protein product
Gene Aliases: CACP; HAPO; JCAP; MSF; PRG4; SZP
UniProt ID: (Human) Q92954
Entrez Gene ID: (Human) 10216
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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