|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human lymphotatin|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-2 ug/ml|
|Functional Assay (FN)||1.7 - 3 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5 ug/ml|
|Western Blot (WB)||0.1 - 0.2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections.
Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended. Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles.
Lymphotactin, otherwise known as XCL1, is a member of the C-chemokine family that uniquely acts as a chemoattractant for lymphocytes, but not monocytes or neutrophils, suggesting an important regulatory role in lymphocyte trafficking and inflammation. Lymphotactin, shown to signal through chemokine XC receptor 1 (XCR1), is detected at the highest level in the spleen and at low levels in the lungs, small intestine and colon, and is abundantly and selectively expressed by activated T cells, in particular CD8+ T cells, and is also produced by NK cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.